Developmental arrest and mouse antral not-surrounded nucleolus oocytes

Manuela Monti, Mario Zanoni, Alberto Calligaro, Minoru Ko, Pierluigi Mauri, Carlo Alberto Redi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The antral compartment in the ovary consists of two populations of oocytes that differ by their ability to resume meiosis and to develop to the blastocyst stage. For reasons still not entirely clear, antral oocytes termed surrounded nucleolus (SN; 70% of the population of antral oocytes) develop to the blastocyst stage, whereas those called not-surrounded nucleolus (NSN) arrest at two cells. We profiled transcriptomic, proteomic, and morphological characteristics of antral oocytes and observed that NSN oocyte arrest is associated with lack of cytoplasmic lattices coincident with reduced expression of MATER and ribosomal proteins. Cytoplasmic lattices have been shown to store maternally derived mRNA and ribosomes in mammalian oocytes and embryos, and MATER has been shown to be required for cytoplasmic lattice formation. Thus, we isolated antral oocytes from a Matertm/tm mouse and we observed that 84% of oocytes are of the NSN type. Our results provide the first molecular evidence to account for inability of NSN-derived embryos to progress beyond the two-cell stage; these results may be relevant to naturally occurring preimplantation embryo demise in mammals.

Original languageEnglish
Article number2
JournalBiology of Reproduction
Volume88
Issue number1
DOIs
Publication statusPublished - 2013 Jan
Externally publishedYes

Fingerprint

Oocytes
Blastocyst
Mammalian Embryo
Antral
Ribosomal Proteins
Meiosis
Ribosomes
Proteomics
Population
Mammals
Ovary
Embryonic Structures
Messenger RNA

Keywords

  • Cytoplasmic lattices
  • Mater
  • Oocyte maturation
  • Oocyte to embryo transition
  • Oogenesis
  • Preimplantation embryo development

ASJC Scopus subject areas

  • Cell Biology

Cite this

Developmental arrest and mouse antral not-surrounded nucleolus oocytes. / Monti, Manuela; Zanoni, Mario; Calligaro, Alberto; Ko, Minoru; Mauri, Pierluigi; Redi, Carlo Alberto.

In: Biology of Reproduction, Vol. 88, No. 1, 2, 01.2013.

Research output: Contribution to journalArticle

Monti, Manuela ; Zanoni, Mario ; Calligaro, Alberto ; Ko, Minoru ; Mauri, Pierluigi ; Redi, Carlo Alberto. / Developmental arrest and mouse antral not-surrounded nucleolus oocytes. In: Biology of Reproduction. 2013 ; Vol. 88, No. 1.
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