Diabetes and tumor formation in transgenic mice expressing Reg I

Takashi Yamaoka, Kenji Yoshino, Taketo Yamada, Chiyoko Idehara, Mohammad O. Hoque, Maki Moritani, Katsuhiko Yoshimoto, Jun ich Hata, Mitsuo Itakura

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25 Citations (Scopus)

Abstract

To examine the effect of overexpressed regenerating gene (Reg) I on pancreatic β-cells, we generated transgenic mice expressing Reg I in islets (Reg-Tg mice). Three lines of Reg-Tg mice were established. In line-1 Reg-Tg mice, the expression level of Reg I mRNA in islets was 7 times higher than those in lines 2 and 3 of Reg-Tg mice, and line 1 mice developed diabetes by apoptosis of β-cells, as well as various malignant tumors. In addition to the decrease in β-cells, compensatory islet regeneration and proliferation of ductal epithelial cells were observed in llne-1 Reg-Tg mice. Because Reg I protein was secreted primarily into pancreatic ducts from acinar cells, it may primarily stimulate the proliferation of ductal epithelial cells, and not β-cells, and their differentiation into islets. Moreover, the tumor-promoting activity of Reg I protein should be considered for its possible clinical applications. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)368-376
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number2
DOIs
Publication statusPublished - 2000 Nov 19

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Keywords

  • Apoptosis
  • Neoplasm
  • Pancreatic islet
  • Reg I
  • Regeneration
  • Transgenic mouse

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yamaoka, T., Yoshino, K., Yamada, T., Idehara, C., Hoque, M. O., Moritani, M., Yoshimoto, K., Hata, J. I., & Itakura, M. (2000). Diabetes and tumor formation in transgenic mice expressing Reg I. Biochemical and Biophysical Research Communications, 278(2), 368-376. https://doi.org/10.1006/bbrc.2000.3813