Diabetes and tumor formation in transgenic mice expressing Reg I

Takashi Yamaoka, Kenji Yoshino, Taketo Yamada, Chiyoko Idehara, Mohammad O. Hoque, Maki Moritani, Katsuhiko Yoshimoto, Jun ich Hata, Mitsuo Itakura

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

To examine the effect of overexpressed regenerating gene (Reg) I on pancreatic β-cells, we generated transgenic mice expressing Reg I in islets (Reg-Tg mice). Three lines of Reg-Tg mice were established. In line-1 Reg-Tg mice, the expression level of Reg I mRNA in islets was 7 times higher than those in lines 2 and 3 of Reg-Tg mice, and line 1 mice developed diabetes by apoptosis of β-cells, as well as various malignant tumors. In addition to the decrease in β-cells, compensatory islet regeneration and proliferation of ductal epithelial cells were observed in llne-1 Reg-Tg mice. Because Reg I protein was secreted primarily into pancreatic ducts from acinar cells, it may primarily stimulate the proliferation of ductal epithelial cells, and not β-cells, and their differentiation into islets. Moreover, the tumor-promoting activity of Reg I protein should be considered for its possible clinical applications. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)368-376
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number2
DOIs
Publication statusPublished - 2000 Nov 19

Fingerprint

Medical problems
Transgenic Mice
Tumors
Genes
Neoplasms
Epithelial Cells
Acinar Cells
Pancreatic Ducts
Islets of Langerhans
Regeneration
Cell Differentiation
Proteins
Ducts
Apoptosis
Messenger RNA

Keywords

  • Apoptosis
  • Neoplasm
  • Pancreatic islet
  • Reg I
  • Regeneration
  • Transgenic mouse

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yamaoka, T., Yoshino, K., Yamada, T., Idehara, C., Hoque, M. O., Moritani, M., ... Itakura, M. (2000). Diabetes and tumor formation in transgenic mice expressing Reg I. Biochemical and Biophysical Research Communications, 278(2), 368-376. https://doi.org/10.1006/bbrc.2000.3813

Diabetes and tumor formation in transgenic mice expressing Reg I. / Yamaoka, Takashi; Yoshino, Kenji; Yamada, Taketo; Idehara, Chiyoko; Hoque, Mohammad O.; Moritani, Maki; Yoshimoto, Katsuhiko; Hata, Jun ich; Itakura, Mitsuo.

In: Biochemical and Biophysical Research Communications, Vol. 278, No. 2, 19.11.2000, p. 368-376.

Research output: Contribution to journalArticle

Yamaoka, T, Yoshino, K, Yamada, T, Idehara, C, Hoque, MO, Moritani, M, Yoshimoto, K, Hata, JI & Itakura, M 2000, 'Diabetes and tumor formation in transgenic mice expressing Reg I', Biochemical and Biophysical Research Communications, vol. 278, no. 2, pp. 368-376. https://doi.org/10.1006/bbrc.2000.3813
Yamaoka T, Yoshino K, Yamada T, Idehara C, Hoque MO, Moritani M et al. Diabetes and tumor formation in transgenic mice expressing Reg I. Biochemical and Biophysical Research Communications. 2000 Nov 19;278(2):368-376. https://doi.org/10.1006/bbrc.2000.3813
Yamaoka, Takashi ; Yoshino, Kenji ; Yamada, Taketo ; Idehara, Chiyoko ; Hoque, Mohammad O. ; Moritani, Maki ; Yoshimoto, Katsuhiko ; Hata, Jun ich ; Itakura, Mitsuo. / Diabetes and tumor formation in transgenic mice expressing Reg I. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 278, No. 2. pp. 368-376.
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