Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification

Yae Kanai, Masahiro Gotoh, Eri Arai, Saori Wakai-Ushijima, Nobuyoshi Hiraoka, Tomoo Kosuge, Fumie Hosoda, Tatsuhiro Shibata, Tadashi Kondo, Sana Yokoi, Issei Imoto, Johji Inazawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tissue (T) from noncancerous pancreatic tissue in the learning cohort with a specificity of 100, were identified. Using criteria that combined the 12 BAC clones, T-samples were diagnosed as cancers with 100 sensitivity and specificity in both the learning and validation cohorts. DNA methylation status on 11 of the BAC clones, which was able to discriminate patients showing early relapse from those with no relapse in the learning cohort with 100 specificity, was correlated with the recurrence-free and overall survival rates in the validation cohort and was an independent prognostic factor by multivariate analysis. Genome-wide DNA methylation profiling may provide optimal diagnostic markers and prognostic indicators for patients with PCs.

Original languageEnglish
Article number780836
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Bacterial Artificial Chromosomes
CpG Islands
DNA Fingerprinting
DNA Methylation
Chromosomes
Amplification
Pancreas
Adenocarcinoma
Genes
Genome
Clone Cells
Learning
Tissue
Recurrence
Statistical Factor Analysis
Multivariate Analysis
Survival Rate
Sensitivity and Specificity
Neoplasms

ASJC Scopus subject areas

  • Biotechnology
  • Medicine(all)
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification. / Kanai, Yae; Gotoh, Masahiro; Arai, Eri; Wakai-Ushijima, Saori; Hiraoka, Nobuyoshi; Kosuge, Tomoo; Hosoda, Fumie; Shibata, Tatsuhiro; Kondo, Tadashi; Yokoi, Sana; Imoto, Issei; Inazawa, Johji.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 780836, 2011.

Research output: Contribution to journalArticle

Kanai, Yae ; Gotoh, Masahiro ; Arai, Eri ; Wakai-Ushijima, Saori ; Hiraoka, Nobuyoshi ; Kosuge, Tomoo ; Hosoda, Fumie ; Shibata, Tatsuhiro ; Kondo, Tadashi ; Yokoi, Sana ; Imoto, Issei ; Inazawa, Johji. / Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification. In: Journal of Biomedicine and Biotechnology. 2011 ; Vol. 2011.
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AU - Wakai-Ushijima, Saori

AU - Hiraoka, Nobuyoshi

AU - Kosuge, Tomoo

AU - Hosoda, Fumie

AU - Shibata, Tatsuhiro

AU - Kondo, Tadashi

AU - Yokoi, Sana

AU - Imoto, Issei

AU - Inazawa, Johji

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