Diagnostic clinical application of two-color fluorescence in situ hybridization that detects chromosome 1 and 17 alterations to direct touch smear and liquid-based thin-layer cytologic preparations of endometrial cancers

Nobuyuki Susumu, Daisuke Aoki, T. Noda, Y. Nagashima, T. Hirao, Y. Tamada, Kouji Banno, A. Suzuki, N. Suzuki, H. Tsuda, J. Inazawa, S. Nozawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We performed two-color fluorescence in situ hybridization (FISH) on direct touch smears and liquid-based thin-layer (ThinPrep) cytological preparations of endometrial tumors to detect alterations of chromosome 1 and 17 that present with high incidence in endometrial cancers. The DNA probes used for two-color FISH analysis were a combination of the probes designed for 17cen (cCI17-321) and 17p13.3 (D17S34), and a combination of the probes designed for Iq12 (D1Z1) and 1p36 (cCI1-5335). Numerical or structural alterations of chromosome 1 and/or 17 were detected in 95% (19 of 20 cases) of the direct touch smears obtained from endometrial cancer, while these alterations were also detected in 93% (12 of 13 cases) of samples obtained from grade 1 endometrioid adenocarcinoma cases, including three cases that could not be diagnosed as positive by conventional Papanicolaou cytopathologic staining. Using ThinPrep cytopathologic preparations, numerical or structural abnormalities were found in 26 (90%) and five (100%) cases, respectively, of samples obtained transcervically from 29 endometrial cancer and five atypical endometrial hyperplasia cases. Therefore, two-color FISH may be a useful diagnostic method for endometrial adenocarcinoma and premalignant lesions that demonstrate only slight cellular atypia in conventional cytopathologic preparations.

Original languageEnglish
Pages (from-to)70-80
Number of pages11
JournalInternational Journal of Gynecological Cancer
Volume15
Issue number1
DOIs
Publication statusPublished - 2005 Jan

Fingerprint

Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 1
Touch
Endometrial Neoplasms
Fluorescence In Situ Hybridization
Color
Endometrioid Carcinoma
Endometrial Hyperplasia
DNA Probes
Adenocarcinoma
Staining and Labeling
Incidence
Neoplasms

Keywords

  • Cytological diagnosis
  • Endometrial cancer
  • Liquid-based cytology
  • Two-color FISH

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology
  • Cancer Research

Cite this

Diagnostic clinical application of two-color fluorescence in situ hybridization that detects chromosome 1 and 17 alterations to direct touch smear and liquid-based thin-layer cytologic preparations of endometrial cancers. / Susumu, Nobuyuki; Aoki, Daisuke; Noda, T.; Nagashima, Y.; Hirao, T.; Tamada, Y.; Banno, Kouji; Suzuki, A.; Suzuki, N.; Tsuda, H.; Inazawa, J.; Nozawa, S.

In: International Journal of Gynecological Cancer, Vol. 15, No. 1, 01.2005, p. 70-80.

Research output: Contribution to journalArticle

@article{0754c5e7e58246d8a4a669b5070c77e6,
title = "Diagnostic clinical application of two-color fluorescence in situ hybridization that detects chromosome 1 and 17 alterations to direct touch smear and liquid-based thin-layer cytologic preparations of endometrial cancers",
abstract = "We performed two-color fluorescence in situ hybridization (FISH) on direct touch smears and liquid-based thin-layer (ThinPrep) cytological preparations of endometrial tumors to detect alterations of chromosome 1 and 17 that present with high incidence in endometrial cancers. The DNA probes used for two-color FISH analysis were a combination of the probes designed for 17cen (cCI17-321) and 17p13.3 (D17S34), and a combination of the probes designed for Iq12 (D1Z1) and 1p36 (cCI1-5335). Numerical or structural alterations of chromosome 1 and/or 17 were detected in 95{\%} (19 of 20 cases) of the direct touch smears obtained from endometrial cancer, while these alterations were also detected in 93{\%} (12 of 13 cases) of samples obtained from grade 1 endometrioid adenocarcinoma cases, including three cases that could not be diagnosed as positive by conventional Papanicolaou cytopathologic staining. Using ThinPrep cytopathologic preparations, numerical or structural abnormalities were found in 26 (90{\%}) and five (100{\%}) cases, respectively, of samples obtained transcervically from 29 endometrial cancer and five atypical endometrial hyperplasia cases. Therefore, two-color FISH may be a useful diagnostic method for endometrial adenocarcinoma and premalignant lesions that demonstrate only slight cellular atypia in conventional cytopathologic preparations.",
keywords = "Cytological diagnosis, Endometrial cancer, Liquid-based cytology, Two-color FISH",
author = "Nobuyuki Susumu and Daisuke Aoki and T. Noda and Y. Nagashima and T. Hirao and Y. Tamada and Kouji Banno and A. Suzuki and N. Suzuki and H. Tsuda and J. Inazawa and S. Nozawa",
year = "2005",
month = "1",
doi = "10.1111/j.1048-891x.2005.14424.x",
language = "English",
volume = "15",
pages = "70--80",
journal = "International Journal of Gynecological Cancer",
issn = "1048-891X",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Diagnostic clinical application of two-color fluorescence in situ hybridization that detects chromosome 1 and 17 alterations to direct touch smear and liquid-based thin-layer cytologic preparations of endometrial cancers

AU - Susumu, Nobuyuki

AU - Aoki, Daisuke

AU - Noda, T.

AU - Nagashima, Y.

AU - Hirao, T.

AU - Tamada, Y.

AU - Banno, Kouji

AU - Suzuki, A.

AU - Suzuki, N.

AU - Tsuda, H.

AU - Inazawa, J.

AU - Nozawa, S.

PY - 2005/1

Y1 - 2005/1

N2 - We performed two-color fluorescence in situ hybridization (FISH) on direct touch smears and liquid-based thin-layer (ThinPrep) cytological preparations of endometrial tumors to detect alterations of chromosome 1 and 17 that present with high incidence in endometrial cancers. The DNA probes used for two-color FISH analysis were a combination of the probes designed for 17cen (cCI17-321) and 17p13.3 (D17S34), and a combination of the probes designed for Iq12 (D1Z1) and 1p36 (cCI1-5335). Numerical or structural alterations of chromosome 1 and/or 17 were detected in 95% (19 of 20 cases) of the direct touch smears obtained from endometrial cancer, while these alterations were also detected in 93% (12 of 13 cases) of samples obtained from grade 1 endometrioid adenocarcinoma cases, including three cases that could not be diagnosed as positive by conventional Papanicolaou cytopathologic staining. Using ThinPrep cytopathologic preparations, numerical or structural abnormalities were found in 26 (90%) and five (100%) cases, respectively, of samples obtained transcervically from 29 endometrial cancer and five atypical endometrial hyperplasia cases. Therefore, two-color FISH may be a useful diagnostic method for endometrial adenocarcinoma and premalignant lesions that demonstrate only slight cellular atypia in conventional cytopathologic preparations.

AB - We performed two-color fluorescence in situ hybridization (FISH) on direct touch smears and liquid-based thin-layer (ThinPrep) cytological preparations of endometrial tumors to detect alterations of chromosome 1 and 17 that present with high incidence in endometrial cancers. The DNA probes used for two-color FISH analysis were a combination of the probes designed for 17cen (cCI17-321) and 17p13.3 (D17S34), and a combination of the probes designed for Iq12 (D1Z1) and 1p36 (cCI1-5335). Numerical or structural alterations of chromosome 1 and/or 17 were detected in 95% (19 of 20 cases) of the direct touch smears obtained from endometrial cancer, while these alterations were also detected in 93% (12 of 13 cases) of samples obtained from grade 1 endometrioid adenocarcinoma cases, including three cases that could not be diagnosed as positive by conventional Papanicolaou cytopathologic staining. Using ThinPrep cytopathologic preparations, numerical or structural abnormalities were found in 26 (90%) and five (100%) cases, respectively, of samples obtained transcervically from 29 endometrial cancer and five atypical endometrial hyperplasia cases. Therefore, two-color FISH may be a useful diagnostic method for endometrial adenocarcinoma and premalignant lesions that demonstrate only slight cellular atypia in conventional cytopathologic preparations.

KW - Cytological diagnosis

KW - Endometrial cancer

KW - Liquid-based cytology

KW - Two-color FISH

UR - http://www.scopus.com/inward/record.url?scp=19944430220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944430220&partnerID=8YFLogxK

U2 - 10.1111/j.1048-891x.2005.14424.x

DO - 10.1111/j.1048-891x.2005.14424.x

M3 - Article

VL - 15

SP - 70

EP - 80

JO - International Journal of Gynecological Cancer

JF - International Journal of Gynecological Cancer

SN - 1048-891X

IS - 1

ER -