Diameter of fluorescent microspheres determines their distribution throughout the cortical watershed area in mice

Naoki Tsukada, Masahiro Katsumata, Koichi Oki, Kazushi Minami, Takato Abe, Shinichi Takahashi, Yoshiaki Itoh, Norihiro Suzuki

Research output: Contribution to journalArticle

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Abstract

A hemodynamic mechanism has long been assumed to play an important role in watershed infarction. In recent years, however, clinical evidence has indicated that an embolic mechanism is involved. The mechanism by which emboli are trapped preferentially in watershed areas remains unclear. In the present study, we developed a mouse embolus model using fluorescent microspheres with different diameters and evaluated the role of the microspheres’ diameters in the generation of a watershed-patterned distribution. We injected fluorescent microspheres of four different diameters (i.e., 13, 24, 40, and 69 μm) into the internal carotid artery of C57BL/6 mice either (1) without ligation of the common carotid artery (normal perfusion pressure model: NPPM) or (2) with ligation of the common carotid artery (low perfusion pressure model: LPPM). Left common carotid artery ligation induced reductions in local cerebral blood flow in both the periphery and the core area of the left middle cerebral artery. A greater reduction in the border-zone area between the left anterior cerebral artery and the middle cerebral artery was also noted. After 24 h, the brains were removed and the distribution of the microspheres in the brain was evaluated using a fluorescence microscope. The 24-μm microspheres were distributed in the watershed area more frequently than the other microsphere sizes (P <.05, ANOVA followed by Tukey's test). Meanwhile, the distribution rates were similar between the NPPM and LPPM models for all microsphere sizes. This study suggested that the distribution pattern of the microspheres was only affected by the microspheres’ diameters.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalBrain Research
Volume1679
DOIs
Publication statusPublished - 2018 Jan 15

Fingerprint

Microspheres
Common Carotid Artery
Ligation
Middle Cerebral Artery
Embolism
Cerebrovascular Circulation
Perfusion
Anterior Cerebral Artery
Pressure
Brain
Internal Carotid Artery
Inbred C57BL Mouse
Infarction
Analysis of Variance
Fluorescence
Hemodynamics

Keywords

  • Carotid stenosis
  • Embolic infarction
  • Fluorescent microsphere
  • Hemodynamic infarction
  • Watershed infarction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Diameter of fluorescent microspheres determines their distribution throughout the cortical watershed area in mice. / Tsukada, Naoki; Katsumata, Masahiro; Oki, Koichi; Minami, Kazushi; Abe, Takato; Takahashi, Shinichi; Itoh, Yoshiaki; Suzuki, Norihiro.

In: Brain Research, Vol. 1679, 15.01.2018, p. 109-115.

Research output: Contribution to journalArticle

Tsukada, Naoki ; Katsumata, Masahiro ; Oki, Koichi ; Minami, Kazushi ; Abe, Takato ; Takahashi, Shinichi ; Itoh, Yoshiaki ; Suzuki, Norihiro. / Diameter of fluorescent microspheres determines their distribution throughout the cortical watershed area in mice. In: Brain Research. 2018 ; Vol. 1679. pp. 109-115.
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