Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa, Yuto Tanaka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1:06 J=cm2. The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

Original languageEnglish
Pages (from-to)2877-2879
Number of pages3
JournalOptics Letters
Volume36
Issue number15
DOIs
Publication statusPublished - 2011 Aug 1

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polystyrene
cells
lasers
dextrans
pulses
antibodies
fluence
wavelengths
molecules

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Dielectric microsphere mediated transfection using a femtosecond laser. / Terakawa, Mitsuhiro; Tanaka, Yuto.

In: Optics Letters, Vol. 36, No. 15, 01.08.2011, p. 2877-2879.

Research output: Contribution to journalArticle

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