Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa, Yuto Tanaka

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    24 Citations (Scopus)

    Abstract

    We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1:06 J=cm2. The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

    Original languageEnglish
    Pages (from-to)2877-2879
    Number of pages3
    JournalOptics Letters
    Volume36
    Issue number15
    DOIs
    Publication statusPublished - 2011 Aug 1

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    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics

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