Dielectrophoretic micro-organization of chondrocytes to regenerate mechanically anisotropic cartilaginous tissue

Yoshitaka Takeuchi, Shogo Miyata

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, many studies have focused on the repair and regeneration of damaged articular cartilage using tissue engineering. In tissue engineering therapy, cells are cultured in vitro to create a three-dimensional (3-D) tissue designed to replace the damaged cartilage. Although tissue engineering is a useful approach to regenerating cartilage, mechanical anisotropy has not been reconstructed from a cellular organization level. This study aims to create mechanically anisotropic cartilaginous tissue using dielectrophoretic cell patterning and gel-sheet lamination. Bovine chondrocytes were patterned in a hydrogel to form line-array cell clusters via negative dielectrophoresis (DEP). The results indicate that the embedded chondrocytes remained viable and reconstructed cartilaginous tissue along the patterned cell array. Moreover, the agarose gel, in which chondrocytes were patterned, demonstrated mechanical anisotropy. In summary, our DEP cell patterning and gel-sheet lamination techniques would be useful for reconstructing mechanically anisotropic cartilage tissues.

Original languageEnglish
Article number1098
JournalMicromachines
Volume12
Issue number9
DOIs
Publication statusPublished - 2021 Sep

Keywords

  • Cell patterning
  • Chondrocyte
  • Dielectrophoresis
  • Mechanical anisotropy
  • Tissue engineering

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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