Dielectrophoretic sorting system for screening of NAD(P)-dependent oxidoreductases.

Yuki Kanai, Shota Shimokihara, Shunya Shitara, Ryo Oyobiki, Takeshi Watanabe, Yasuaki Einaga, Kunihiko Wakamatsu, Yoshinori Matsumoto, Kei Fujiwara, Nobuhide Doi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we report the development of a flow injection analysis system based on electrochemical measurements and dielectrophoresis for screening of NAD(P)-dependent oxidoreductases. In this system, the activity of enzyme which is immobilized on microbeads together with its encoding DNA can be measured with a boron-doped diamond (BDD) electrode in each compartment (~30 nL) of the microfluidic system, and the large droplets (~30 nL) containing genes of enzymes with higher activity can be recovered by dielectrophoretic sorting.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1383-1384
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020 Jan 1
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

Keywords

  • Boron-doped diamond electrode
  • Cell-free protein synthesis
  • Dielectrophoresis
  • Directed evolution
  • High-throughput screening

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Kanai, Y., Shimokihara, S., Shitara, S., Oyobiki, R., Watanabe, T., Einaga, Y., Wakamatsu, K., Matsumoto, Y., Fujiwara, K., & Doi, N. (2020). Dielectrophoretic sorting system for screening of NAD(P)-dependent oxidoreductases. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 1383-1384). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.