Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor

Midori Awazu, Aida Yared, Larry L. Swift, Richard L. Hoover, Iekuni Ichikawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Modification of dietary fatty acid (FA) has been shown to affect the incidence of hypertension and coronary artery disease. We studied whether these effects involve changes in the receptor characteristics of vasoactive substance. Characteristics of atrial natriuretic peptide (ANP) receptors were examined in glomeruli isolated from rats fed a diet containing 5% in weight ω6, 5% ω3, 20% ω6, 20% ω3 polyunsaturated FA or 20% saturated FA (SFA) for >4 weeks. The FA composition of phospholipids in isolated glomeruli showed an elevation in 20:4 ω6 (arachidonic acid, AA) in 5% ω6, 20% ω6 and 20% SFA, and elevations in 20:5 ω3 (eicosapentaenoic acid, EPA) in 5% ω3 and 20% ω3 groups. The radioligand binding study revealed: (1) in 20% FA group, receptor density (Ro, fmol/mg prot) of ANP was significantly decreased compared to 5% group (262 ±13, n = 8 to 120 ± 13, n = 12) without changes in equilibrium dissociation constant (KD), (2) among high FA (20%) groups, type of FA was essential for determining Ro; higher ω6 was associated with a lower ANP Ro (177 ± 11 vs. 103 ± 3 fmol/mg prot, P < 0.05) and KD (0.43 ± .04 vs. 0.27 ± .02 nM, P < 0.05). To examine whether the alteration in receptor characterisitics is mediated by FA, effects of FA were examined in vitro. In cultured mesangial cells, AA, but not EPA, decreased Ro of ANP receptors (48.7 ± 4.8% of control, P < 0.05) without affecting KD. AA-induced down-regulation of ANP receptor was not accompanied by changes in ANP-induced cGMP generation. These results from ex vivo and in vitro studies suggest that dietary FA modulates the glomerular C receptor of ANP. The observed effect of FA on glomerular ANP receptor characteristics raises the possibility of the existence of a novel mechanism through which dietary FA intake affects the cardiovascular physiology and pathophysiology through affecting the receptor characterisitcs of vasoactive substances.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalKidney International
Volume42
Issue number2
Publication statusPublished - 1992 Aug
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor Receptors
Fatty Acids
Atrial Natriuretic Factor
Arachidonic Acid
Eicosapentaenoic Acid
Cardiovascular Physiological Phenomena
Diet Therapy
Essential Fatty Acids
Mesangial Cells
Unsaturated Fatty Acids
Coronary Artery Disease
Cultured Cells
Phospholipids
Down-Regulation

ASJC Scopus subject areas

  • Nephrology

Cite this

Awazu, M., Yared, A., Swift, L. L., Hoover, R. L., & Ichikawa, I. (1992). Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor. Kidney International, 42(2), 265-271.

Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor. / Awazu, Midori; Yared, Aida; Swift, Larry L.; Hoover, Richard L.; Ichikawa, Iekuni.

In: Kidney International, Vol. 42, No. 2, 08.1992, p. 265-271.

Research output: Contribution to journalArticle

Awazu, M, Yared, A, Swift, LL, Hoover, RL & Ichikawa, I 1992, 'Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor', Kidney International, vol. 42, no. 2, pp. 265-271.
Awazu M, Yared A, Swift LL, Hoover RL, Ichikawa I. Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor. Kidney International. 1992 Aug;42(2):265-271.
Awazu, Midori ; Yared, Aida ; Swift, Larry L. ; Hoover, Richard L. ; Ichikawa, Iekuni. / Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor. In: Kidney International. 1992 ; Vol. 42, No. 2. pp. 265-271.
@article{aa72de3793df427b893fb006754440e7,
title = "Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor",
abstract = "Modification of dietary fatty acid (FA) has been shown to affect the incidence of hypertension and coronary artery disease. We studied whether these effects involve changes in the receptor characteristics of vasoactive substance. Characteristics of atrial natriuretic peptide (ANP) receptors were examined in glomeruli isolated from rats fed a diet containing 5{\%} in weight ω6, 5{\%} ω3, 20{\%} ω6, 20{\%} ω3 polyunsaturated FA or 20{\%} saturated FA (SFA) for >4 weeks. The FA composition of phospholipids in isolated glomeruli showed an elevation in 20:4 ω6 (arachidonic acid, AA) in 5{\%} ω6, 20{\%} ω6 and 20{\%} SFA, and elevations in 20:5 ω3 (eicosapentaenoic acid, EPA) in 5{\%} ω3 and 20{\%} ω3 groups. The radioligand binding study revealed: (1) in 20{\%} FA group, receptor density (Ro, fmol/mg prot) of ANP was significantly decreased compared to 5{\%} group (262 ±13, n = 8 to 120 ± 13, n = 12) without changes in equilibrium dissociation constant (KD), (2) among high FA (20{\%}) groups, type of FA was essential for determining Ro; higher ω6 was associated with a lower ANP Ro (177 ± 11 vs. 103 ± 3 fmol/mg prot, P < 0.05) and KD (0.43 ± .04 vs. 0.27 ± .02 nM, P < 0.05). To examine whether the alteration in receptor characterisitics is mediated by FA, effects of FA were examined in vitro. In cultured mesangial cells, AA, but not EPA, decreased Ro of ANP receptors (48.7 ± 4.8{\%} of control, P < 0.05) without affecting KD. AA-induced down-regulation of ANP receptor was not accompanied by changes in ANP-induced cGMP generation. These results from ex vivo and in vitro studies suggest that dietary FA modulates the glomerular C receptor of ANP. The observed effect of FA on glomerular ANP receptor characteristics raises the possibility of the existence of a novel mechanism through which dietary FA intake affects the cardiovascular physiology and pathophysiology through affecting the receptor characterisitcs of vasoactive substances.",
author = "Midori Awazu and Aida Yared and Swift, {Larry L.} and Hoover, {Richard L.} and Iekuni Ichikawa",
year = "1992",
month = "8",
language = "English",
volume = "42",
pages = "265--271",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor

AU - Awazu, Midori

AU - Yared, Aida

AU - Swift, Larry L.

AU - Hoover, Richard L.

AU - Ichikawa, Iekuni

PY - 1992/8

Y1 - 1992/8

N2 - Modification of dietary fatty acid (FA) has been shown to affect the incidence of hypertension and coronary artery disease. We studied whether these effects involve changes in the receptor characteristics of vasoactive substance. Characteristics of atrial natriuretic peptide (ANP) receptors were examined in glomeruli isolated from rats fed a diet containing 5% in weight ω6, 5% ω3, 20% ω6, 20% ω3 polyunsaturated FA or 20% saturated FA (SFA) for >4 weeks. The FA composition of phospholipids in isolated glomeruli showed an elevation in 20:4 ω6 (arachidonic acid, AA) in 5% ω6, 20% ω6 and 20% SFA, and elevations in 20:5 ω3 (eicosapentaenoic acid, EPA) in 5% ω3 and 20% ω3 groups. The radioligand binding study revealed: (1) in 20% FA group, receptor density (Ro, fmol/mg prot) of ANP was significantly decreased compared to 5% group (262 ±13, n = 8 to 120 ± 13, n = 12) without changes in equilibrium dissociation constant (KD), (2) among high FA (20%) groups, type of FA was essential for determining Ro; higher ω6 was associated with a lower ANP Ro (177 ± 11 vs. 103 ± 3 fmol/mg prot, P < 0.05) and KD (0.43 ± .04 vs. 0.27 ± .02 nM, P < 0.05). To examine whether the alteration in receptor characterisitics is mediated by FA, effects of FA were examined in vitro. In cultured mesangial cells, AA, but not EPA, decreased Ro of ANP receptors (48.7 ± 4.8% of control, P < 0.05) without affecting KD. AA-induced down-regulation of ANP receptor was not accompanied by changes in ANP-induced cGMP generation. These results from ex vivo and in vitro studies suggest that dietary FA modulates the glomerular C receptor of ANP. The observed effect of FA on glomerular ANP receptor characteristics raises the possibility of the existence of a novel mechanism through which dietary FA intake affects the cardiovascular physiology and pathophysiology through affecting the receptor characterisitcs of vasoactive substances.

AB - Modification of dietary fatty acid (FA) has been shown to affect the incidence of hypertension and coronary artery disease. We studied whether these effects involve changes in the receptor characteristics of vasoactive substance. Characteristics of atrial natriuretic peptide (ANP) receptors were examined in glomeruli isolated from rats fed a diet containing 5% in weight ω6, 5% ω3, 20% ω6, 20% ω3 polyunsaturated FA or 20% saturated FA (SFA) for >4 weeks. The FA composition of phospholipids in isolated glomeruli showed an elevation in 20:4 ω6 (arachidonic acid, AA) in 5% ω6, 20% ω6 and 20% SFA, and elevations in 20:5 ω3 (eicosapentaenoic acid, EPA) in 5% ω3 and 20% ω3 groups. The radioligand binding study revealed: (1) in 20% FA group, receptor density (Ro, fmol/mg prot) of ANP was significantly decreased compared to 5% group (262 ±13, n = 8 to 120 ± 13, n = 12) without changes in equilibrium dissociation constant (KD), (2) among high FA (20%) groups, type of FA was essential for determining Ro; higher ω6 was associated with a lower ANP Ro (177 ± 11 vs. 103 ± 3 fmol/mg prot, P < 0.05) and KD (0.43 ± .04 vs. 0.27 ± .02 nM, P < 0.05). To examine whether the alteration in receptor characterisitics is mediated by FA, effects of FA were examined in vitro. In cultured mesangial cells, AA, but not EPA, decreased Ro of ANP receptors (48.7 ± 4.8% of control, P < 0.05) without affecting KD. AA-induced down-regulation of ANP receptor was not accompanied by changes in ANP-induced cGMP generation. These results from ex vivo and in vitro studies suggest that dietary FA modulates the glomerular C receptor of ANP. The observed effect of FA on glomerular ANP receptor characteristics raises the possibility of the existence of a novel mechanism through which dietary FA intake affects the cardiovascular physiology and pathophysiology through affecting the receptor characterisitcs of vasoactive substances.

UR - http://www.scopus.com/inward/record.url?scp=0026776951&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026776951&partnerID=8YFLogxK

M3 - Article

C2 - 1328748

AN - SCOPUS:0026776951

VL - 42

SP - 265

EP - 271

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 2

ER -