Difference in autologous blood transfusion-induced inflammatory responses between acute normovolemic hemodilution and preoperative donation

Yoshifumi Kotake, Michiko Yamamoto, Midori Matsumoto, Takashige Yamada, Hiromasa Nagata, Hiroshi Morisaki, Junzo Takeda

Research output: Contribution to journalArticle

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Abstract

Purpose: The inflammatory response triggered by transfusion is implicated in the pathophysiology of transfusion-related immunomodulation. The authors hypothesized that two distinctive autotransfusion methods, acute normovolemic hemodilution (ANH) and preoperative donation (PD), have different influences on both inflammatory mediator generation during storage and the inflammatory response after a transfusion. The purpose of this study was to compare the plasma concentrations of neutrophil elastase (NE), interleukin (IL)-6, IL-8, and IL-10 in patients who underwent either of these two autologous transfusion methods. Methods: With institutional review board approval, the plasma concentrations of the above inflammatory mediators were determined in 23 patients with ANH and 8 patients with PD at the following time points: after anesthetic induction, at the end of the operation, and the morning of postoperative day 1. The concentrations of these inflammatory mediators were also measured in the donated blood obtained by either ANH or PD before retransfusion. Results: The mean storage durations were 3.7 h and 6.1 days for ANH and PD, respectively. Higher concentrations of NE and IL-10 were detected in the PD blood than in the ANH blood. Long duration of storage and/or low temperature may have been responsible for the increased NE and IL-10 concentrations in the PD blood. However, the difference between the two groups in the extent of increased plasma concentrations of these inflammatory mediators was not statistically significant. Conclusion: Inflammatory mediators were significantly increased in PD blood during storage compared to the blood obtained by ANH. However, their effects on the inflammatory response elicited in the recipients were not significantly different.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalJournal of Anesthesia
Volume23
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

Autologous Blood Transfusions
Hemodilution
Leukocyte Elastase
Blood Donors
Interleukin-10
Immunomodulation
Research Ethics Committees
Interleukin-8
Anesthetics
Interleukin-6
Temperature

Keywords

  • Acute normovolemic hemodilution
  • HES 70/0.5
  • Interleukin
  • Neutrophil elastase
  • Preoperative donation

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Difference in autologous blood transfusion-induced inflammatory responses between acute normovolemic hemodilution and preoperative donation. / Kotake, Yoshifumi; Yamamoto, Michiko; Matsumoto, Midori; Yamada, Takashige; Nagata, Hiromasa; Morisaki, Hiroshi; Takeda, Junzo.

In: Journal of Anesthesia, Vol. 23, No. 1, 2009, p. 61-66.

Research output: Contribution to journalArticle

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AU - Kotake, Yoshifumi

AU - Yamamoto, Michiko

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AU - Yamada, Takashige

AU - Nagata, Hiromasa

AU - Morisaki, Hiroshi

AU - Takeda, Junzo

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N2 - Purpose: The inflammatory response triggered by transfusion is implicated in the pathophysiology of transfusion-related immunomodulation. The authors hypothesized that two distinctive autotransfusion methods, acute normovolemic hemodilution (ANH) and preoperative donation (PD), have different influences on both inflammatory mediator generation during storage and the inflammatory response after a transfusion. The purpose of this study was to compare the plasma concentrations of neutrophil elastase (NE), interleukin (IL)-6, IL-8, and IL-10 in patients who underwent either of these two autologous transfusion methods. Methods: With institutional review board approval, the plasma concentrations of the above inflammatory mediators were determined in 23 patients with ANH and 8 patients with PD at the following time points: after anesthetic induction, at the end of the operation, and the morning of postoperative day 1. The concentrations of these inflammatory mediators were also measured in the donated blood obtained by either ANH or PD before retransfusion. Results: The mean storage durations were 3.7 h and 6.1 days for ANH and PD, respectively. Higher concentrations of NE and IL-10 were detected in the PD blood than in the ANH blood. Long duration of storage and/or low temperature may have been responsible for the increased NE and IL-10 concentrations in the PD blood. However, the difference between the two groups in the extent of increased plasma concentrations of these inflammatory mediators was not statistically significant. Conclusion: Inflammatory mediators were significantly increased in PD blood during storage compared to the blood obtained by ANH. However, their effects on the inflammatory response elicited in the recipients were not significantly different.

AB - Purpose: The inflammatory response triggered by transfusion is implicated in the pathophysiology of transfusion-related immunomodulation. The authors hypothesized that two distinctive autotransfusion methods, acute normovolemic hemodilution (ANH) and preoperative donation (PD), have different influences on both inflammatory mediator generation during storage and the inflammatory response after a transfusion. The purpose of this study was to compare the plasma concentrations of neutrophil elastase (NE), interleukin (IL)-6, IL-8, and IL-10 in patients who underwent either of these two autologous transfusion methods. Methods: With institutional review board approval, the plasma concentrations of the above inflammatory mediators were determined in 23 patients with ANH and 8 patients with PD at the following time points: after anesthetic induction, at the end of the operation, and the morning of postoperative day 1. The concentrations of these inflammatory mediators were also measured in the donated blood obtained by either ANH or PD before retransfusion. Results: The mean storage durations were 3.7 h and 6.1 days for ANH and PD, respectively. Higher concentrations of NE and IL-10 were detected in the PD blood than in the ANH blood. Long duration of storage and/or low temperature may have been responsible for the increased NE and IL-10 concentrations in the PD blood. However, the difference between the two groups in the extent of increased plasma concentrations of these inflammatory mediators was not statistically significant. Conclusion: Inflammatory mediators were significantly increased in PD blood during storage compared to the blood obtained by ANH. However, their effects on the inflammatory response elicited in the recipients were not significantly different.

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