Abstract
The histological observation that well-differentiated cancer cells in early hepatocellular carcinoma (HCC) invade portal tracts and/or fibrous hands and that these fibrous tissues then disappear suggests the participation of matrix metalloproteinase-1 (MMP-1) in the degradation of fibrous tissue. To confirm this hypothesis, the authors investigated the localization of both the MMP-1 protein and its messenger RNA (mRNA) in early HCC immunohistochemically and by in situ hybridization using complementary DNA (cDNA) and synthetic antisense probe of MMP-1; they then compared the results with those in advanced HCC. MMP-1 gene transcripts and protein were observed in well-differentiated cancer cells of early HCC but not in moderately or poorly differentiated cancer cells. Thus, cancer cells producing MMP-1 in early HCC may destroy the portal tract tissue adjacent to the cancer lesion and/or the fibrous band of cirrhosis. These results seem to have demonstrated a difference in the mechanism of cancer growth and invasion between early and advanced HCCs.
| Original language | English |
|---|---|
| Pages (from-to) | 580-584 |
| Number of pages | 5 |
| Journal | Hepatology |
| Volume | 25 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1997 |
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ASJC Scopus subject areas
- Hepatology
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Difference in gene expression for matrix metalloproteinase-1 between early and advanced hepatocellular carcinomas. / Okazaki, Isao; Wada, Norihito; Nakano, Masayuki; Saito, Akiko; Takasaki, Ken; Doi, Mikio; Kameyama, Kaori; Otani, Yoshihide; Kubochi, Kiyoshi; Niioka, Maki; Watanabe, Tetsu; Maruyama, Katsuya.
In: Hepatology, Vol. 25, No. 3, 1997, p. 580-584.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Difference in gene expression for matrix metalloproteinase-1 between early and advanced hepatocellular carcinomas
AU - Okazaki, Isao
AU - Wada, Norihito
AU - Nakano, Masayuki
AU - Saito, Akiko
AU - Takasaki, Ken
AU - Doi, Mikio
AU - Kameyama, Kaori
AU - Otani, Yoshihide
AU - Kubochi, Kiyoshi
AU - Niioka, Maki
AU - Watanabe, Tetsu
AU - Maruyama, Katsuya
PY - 1997
Y1 - 1997
N2 - The histological observation that well-differentiated cancer cells in early hepatocellular carcinoma (HCC) invade portal tracts and/or fibrous hands and that these fibrous tissues then disappear suggests the participation of matrix metalloproteinase-1 (MMP-1) in the degradation of fibrous tissue. To confirm this hypothesis, the authors investigated the localization of both the MMP-1 protein and its messenger RNA (mRNA) in early HCC immunohistochemically and by in situ hybridization using complementary DNA (cDNA) and synthetic antisense probe of MMP-1; they then compared the results with those in advanced HCC. MMP-1 gene transcripts and protein were observed in well-differentiated cancer cells of early HCC but not in moderately or poorly differentiated cancer cells. Thus, cancer cells producing MMP-1 in early HCC may destroy the portal tract tissue adjacent to the cancer lesion and/or the fibrous band of cirrhosis. These results seem to have demonstrated a difference in the mechanism of cancer growth and invasion between early and advanced HCCs.
AB - The histological observation that well-differentiated cancer cells in early hepatocellular carcinoma (HCC) invade portal tracts and/or fibrous hands and that these fibrous tissues then disappear suggests the participation of matrix metalloproteinase-1 (MMP-1) in the degradation of fibrous tissue. To confirm this hypothesis, the authors investigated the localization of both the MMP-1 protein and its messenger RNA (mRNA) in early HCC immunohistochemically and by in situ hybridization using complementary DNA (cDNA) and synthetic antisense probe of MMP-1; they then compared the results with those in advanced HCC. MMP-1 gene transcripts and protein were observed in well-differentiated cancer cells of early HCC but not in moderately or poorly differentiated cancer cells. Thus, cancer cells producing MMP-1 in early HCC may destroy the portal tract tissue adjacent to the cancer lesion and/or the fibrous band of cirrhosis. These results seem to have demonstrated a difference in the mechanism of cancer growth and invasion between early and advanced HCCs.
UR - http://www.scopus.com/inward/record.url?scp=0031050890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031050890&partnerID=8YFLogxK
U2 - 10.1002/hep.510250315
DO - 10.1002/hep.510250315
M3 - Article
C2 - 9049202
AN - SCOPUS:0031050890
VL - 25
SP - 580
EP - 584
JO - Hepatology
JF - Hepatology
SN - 0270-9139
IS - 3
ER -