Difference in gene expression for matrix metalloproteinase-1 between early and advanced hepatocellular carcinomas

Isao Okazaki, Norihito Wada, Masayuki Nakano, Akiko Saito, Ken Takasaki, Mikio Doi, Kaori Kameyama, Yoshihide Otani, Kiyoshi Kubochi, Maki Niioka, Tetsu Watanabe, Katsuya Maruyama

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Abstract

The histological observation that well-differentiated cancer cells in early hepatocellular carcinoma (HCC) invade portal tracts and/or fibrous hands and that these fibrous tissues then disappear suggests the participation of matrix metalloproteinase-1 (MMP-1) in the degradation of fibrous tissue. To confirm this hypothesis, the authors investigated the localization of both the MMP-1 protein and its messenger RNA (mRNA) in early HCC immunohistochemically and by in situ hybridization using complementary DNA (cDNA) and synthetic antisense probe of MMP-1; they then compared the results with those in advanced HCC. MMP-1 gene transcripts and protein were observed in well-differentiated cancer cells of early HCC but not in moderately or poorly differentiated cancer cells. Thus, cancer cells producing MMP-1 in early HCC may destroy the portal tract tissue adjacent to the cancer lesion and/or the fibrous band of cirrhosis. These results seem to have demonstrated a difference in the mechanism of cancer growth and invasion between early and advanced HCCs.

Original languageEnglish
Pages (from-to)580-584
Number of pages5
JournalHepatology
Volume25
Issue number3
DOIs
Publication statusPublished - 1997

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Matrix Metalloproteinase 1
Hepatocellular Carcinoma
Gene Expression
Neoplasms
Carcinoma in Situ
In Situ Hybridization
Proteins
Fibrosis
Hand
Complementary DNA
Messenger RNA
Growth

ASJC Scopus subject areas

  • Hepatology

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Difference in gene expression for matrix metalloproteinase-1 between early and advanced hepatocellular carcinomas. / Okazaki, Isao; Wada, Norihito; Nakano, Masayuki; Saito, Akiko; Takasaki, Ken; Doi, Mikio; Kameyama, Kaori; Otani, Yoshihide; Kubochi, Kiyoshi; Niioka, Maki; Watanabe, Tetsu; Maruyama, Katsuya.

In: Hepatology, Vol. 25, No. 3, 1997, p. 580-584.

Research output: Contribution to journalArticle

Okazaki, I, Wada, N, Nakano, M, Saito, A, Takasaki, K, Doi, M, Kameyama, K, Otani, Y, Kubochi, K, Niioka, M, Watanabe, T & Maruyama, K 1997, 'Difference in gene expression for matrix metalloproteinase-1 between early and advanced hepatocellular carcinomas', Hepatology, vol. 25, no. 3, pp. 580-584. https://doi.org/10.1002/hep.510250315
Okazaki, Isao ; Wada, Norihito ; Nakano, Masayuki ; Saito, Akiko ; Takasaki, Ken ; Doi, Mikio ; Kameyama, Kaori ; Otani, Yoshihide ; Kubochi, Kiyoshi ; Niioka, Maki ; Watanabe, Tetsu ; Maruyama, Katsuya. / Difference in gene expression for matrix metalloproteinase-1 between early and advanced hepatocellular carcinomas. In: Hepatology. 1997 ; Vol. 25, No. 3. pp. 580-584.
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