Difference of cell lineage expression of haematopoietic progenitor cells in Philadelphia-positive acute lymphoblastic leukaemia and chronic myelogenous leukaemia

K. Kitano, Y. Sato, T. Suda, Y. Miura

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Abstract

It is still difficult to clinically distinguish Philadelphia (Ph1)-positive acute lymphoblastic leukaemia (ALL) from Ph1-positive chronic myelogenous leukaemia (CML) in lymphoid crisis. In this study we tried to discriminate between these two disorders by simultaneous analyses of cell morphology and karyotype in vitro colonies. We studied three patients with Ph1-positive ALL and four with Ph1-positive CML in various phases of the disease. Bone marrow and peripheral blood cells obtained directly from all seven patients showed abnormal karyotypes including Ph1-chromosomes. Normal karyotypes were found in a small proportion of cells from two ALL patients, but none were found in any from the CML patients. The patients' mononuclear cells (MNCs) were plated at 1-5 x 104/ml in semi-solid medium containing methylcellulose plus phytohaemagglutinin-stimulated leucocyte conditioned medium and erythropoietin. After 9-14 d cultivation, granulocyte-macrophage, erythroid and mixed colonies obtained were used for simultaneous analysis of cell morphology and karyotype. Morphological examination showed that these colonies contained neutrophils, eosinophils, basophils, macrophages and/or erythroblasts in various combinations. No lymphoblast collonies were obtained under the culture conditions used. Cytogenetic examination revealed that all metaphase cells observed in colonies obtained from Ph1-positive ALL patient MNCs had a normal karyotype, whereas those in colonies from Ph1-positive CML patient MNCs had abnormal karyotypes, including pH1 chromosomes, suggesting that the difference between the two disorders involved a difference in cell lineage. Our results showed that this method was a practicable method for distinguishing Ph1-positive ALL from Ph1-positive CML in lymphoid crisis.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalBritish Journal of Haematology
Volume70
Issue number1
Publication statusPublished - 1988

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Cell Lineage
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Hematopoietic Stem Cells
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Karyotype
Abnormal Karyotype
Philadelphia Chromosome
Macrophages
Erythroblasts
Methylcellulose
Basophils
Phytohemagglutinins
Metaphase
Conditioned Culture Medium
Erythropoietin
Granulocytes
Eosinophils
Cytogenetics
Blood Cells
Neutrophils

ASJC Scopus subject areas

  • Hematology

Cite this

Difference of cell lineage expression of haematopoietic progenitor cells in Philadelphia-positive acute lymphoblastic leukaemia and chronic myelogenous leukaemia. / Kitano, K.; Sato, Y.; Suda, T.; Miura, Y.

In: British Journal of Haematology, Vol. 70, No. 1, 1988, p. 21-26.

Research output: Contribution to journalArticle

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