Differences in apoptosis induced by anticancer drugs in sublines (SKG-3a, SKG-3b) from a human uterine cervical epidermoid carcinoma

Motoo Takahashi, Shiro Kataoka, Eiichi Kobayashi, Yasuhiro Udagawa, Daisuke Aoki, Shinji Oie, Akiko Kozu, Shiro Nozawa

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Abstract

SKG-3a and SKG-3b are two distinct human uterine cervical epidermoid carcinoma cell lines derived from a single donor. We studied these two closely related cell lines from the standpoint of drug susceptibility. The growth inhibitory effects of cisplatin (CDDP), doxorubicin (ADM), etoposide (VP-16), and paclitaxel (taxol) on SKG-3a and SKG-3b cells assessed by crystalviolet dye uptake assay were almost the same. SKG-3b cells treated with CDDP, ADM, VP-16, and taxol snowed the apoptotic cell death, whereas apoptosis in SKG-3a cells was not induced by these anticancer drugs. Caspase-3 activity was increased only in the SKG-3b cell lysate after treatment with CDDP, ADM, and VP-16 but was not found in the SKG-3a cell lysate. These results indicate that despite growth inhibitory effects of anticancer drugs being almost the same, there may be differences in the common signaling pathways involved in the apoptotic process between SKG-3a and SKG-3b obtained from the same tumor.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalOncology research
Volume11
Issue number2
Publication statusPublished - 1999 Dec 1

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Keywords

  • Anticancer drugs
  • Apoptosis
  • Caspase-3
  • SKG-3a
  • SKG-3b

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Takahashi, M., Kataoka, S., Kobayashi, E., Udagawa, Y., Aoki, D., Oie, S., Kozu, A., & Nozawa, S. (1999). Differences in apoptosis induced by anticancer drugs in sublines (SKG-3a, SKG-3b) from a human uterine cervical epidermoid carcinoma. Oncology research, 11(2), 71-75.