Differences in cytokine gene expression profile between acute and secondary injury in adult rat spinal cord

Masaya Nakamura, Richard A. Houghtling, Linda MacArthur, Barbara M. Bayer, Barbara S. Bregman

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

It is likely that the environment within the injured spinal cord influences the capacity of fetal spinal cord transplants to support axonal growth. We have recently demonstrated that fetal spinal cord transplants and neurotrophin administration support axonal regeneration after spinal cord transection, and that the distance and amount of axonal growth is greater when these treatments are delayed by several weeks after injury (Coumans et al., 2001). In this study, we sought to determine whether differences in inflammatory mediators exist between the acutely injured spinal cord and the spinal cord after a second injury and re-section, which could provide a more favorable environment for the axonal re-growth. The results of this study show a more rapid induction of transforming growth factor (TGF) β1 mRNA expression in the re-injured spinal cord than the acutely injured spinal cord and an attenuation of proinflammatory cytokine mRNA expression. Furthermore, there was a rapid recruitment of activated microglia/macrophages in the degenerating white matter rostral and caudal to the injury but fewer within the lesion site itself. These findings suggest that the augmentation of TGFβ-1 gene expression and the attenuation of pro-inflammatory cytokine gene expression combined with an altered distribution of activated microglia/macrophages in the re-injured spinal cord might create a more favorable milieu for transplants and axonal regrowth as compared to the acutely injured spinal cord.

Original languageEnglish
Pages (from-to)313-325
Number of pages13
JournalExperimental Neurology
Volume184
Issue number1
DOIs
Publication statusPublished - 2003 Nov

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Transcriptome
Spinal Cord
Cytokines
Wounds and Injuries
Microglia
Transforming Growth Factors
Transplants
Growth
Macrophages
Gene Expression
Messenger RNA
Nerve Growth Factors
Spinal Cord Injuries
Regeneration

Keywords

  • Cytokine
  • Inflammatory response
  • Macrophage
  • Microglia
  • Spinal cord injury
  • TGFβ

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Differences in cytokine gene expression profile between acute and secondary injury in adult rat spinal cord. / Nakamura, Masaya; Houghtling, Richard A.; MacArthur, Linda; Bayer, Barbara M.; Bregman, Barbara S.

In: Experimental Neurology, Vol. 184, No. 1, 11.2003, p. 313-325.

Research output: Contribution to journalArticle

Nakamura, Masaya ; Houghtling, Richard A. ; MacArthur, Linda ; Bayer, Barbara M. ; Bregman, Barbara S. / Differences in cytokine gene expression profile between acute and secondary injury in adult rat spinal cord. In: Experimental Neurology. 2003 ; Vol. 184, No. 1. pp. 313-325.
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