Differences in integrin-dependent phagocytosis among three hemocyte subpopulations of the Pacific oyster "Crassostrea gigas"

Kazutaka Terahara, Keisuke G. Takahashi, Akifumi Nakamura, Makoto Osada, Masaki Yoda, Takachika Hiroi, Masatomo Hirasawa, Katsuyoshi Mori

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Integrins play a key role in immunoresponses such as attachment, spreading, and phagocytosis in invertebrate hemocytes. This study was designed to identify integrin expression patterns at the hemocyte subpopulation level, and correlate the expression levels with phagocytic ability. First, we cloned a β integrin from Crassostrea gigas hemocytes and used real-time RT-PCR to analyze the quantitative expression level of its encoding mRNA. The expression level in hyalinocytes was significantly higher than that in granulocytes and agranulocytes. Subsequently, we investigated the phagocytic ability of each subpopulation using anti-α5β1 integrin antibody, and found that phagocytosis of hyalinocytes was inhibited by neutralization with the antibody but enhanced against the antibody-conjugated microspheres. In contrast, phagocytic abilities of granulocytes and agranulocytes showed high and zero levels, respectively, regardless of the antibody. These results suggest that phagocytosis of hyalinocytes is regulated by an integrin-dependent mechanism and that of granulocytes is elicited by other functional receptors.

Original languageEnglish
Pages (from-to)667-683
Number of pages17
JournalDevelopmental and Comparative Immunology
Volume30
Issue number8
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Crassostrea
Hemocytes
Ostreidae
Phagocytosis
Integrins
Granulocytes
Antibodies
Invertebrates
Microspheres
Real-Time Polymerase Chain Reaction
Messenger RNA

Keywords

  • Bivalve
  • Cloning
  • Crassostrea gigas
  • Hemocyte
  • Integrin
  • Pacific oyster
  • Phagocytosis
  • Real-time RT-PCR

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Differences in integrin-dependent phagocytosis among three hemocyte subpopulations of the Pacific oyster "Crassostrea gigas". / Terahara, Kazutaka; Takahashi, Keisuke G.; Nakamura, Akifumi; Osada, Makoto; Yoda, Masaki; Hiroi, Takachika; Hirasawa, Masatomo; Mori, Katsuyoshi.

In: Developmental and Comparative Immunology, Vol. 30, No. 8, 2006, p. 667-683.

Research output: Contribution to journalArticle

Terahara, Kazutaka ; Takahashi, Keisuke G. ; Nakamura, Akifumi ; Osada, Makoto ; Yoda, Masaki ; Hiroi, Takachika ; Hirasawa, Masatomo ; Mori, Katsuyoshi. / Differences in integrin-dependent phagocytosis among three hemocyte subpopulations of the Pacific oyster "Crassostrea gigas". In: Developmental and Comparative Immunology. 2006 ; Vol. 30, No. 8. pp. 667-683.
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