Different contributions of side-chains in β-d-(1 → 3,6)-galactans on intestinal Peyer's patch-immunomodulation by polysaccharides from Astragalus mongholics Bunge

Hiroaki Kiyohara, Taichi Uchida, Manami Takakiwa, Toshiake Matsuzaki, Noriyasu Hada, Tadahiro Takeda, Toshiro Shibata, Haruki Yamada

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Thirteen polysaccharides isolated from an extract of the aerial portions of Astragalus mongholics Bunge demonstrated immunomodulating activity against Peyer's patch immunocompetent cells. Nine of the active polysaccharide fractions were composed of either arabinogalactans, pectic arabinogalactans or pectins. The activities of the arabinogalactans and pectic arabinogalactans were associated with β-d-(1 → 3)-galactan moieties branched with β-d-(1 → 6)-galactooligosaccharide side-chains having degrees of polymerization of 8 or less. Degradation of the β-d-(1 → 3)-galactan or β-d-(1 → 6)-galactosyl side-chains in the arabinogalactans significantly decreased immunomodulating activity. Rhamnogalacturonan I (RG-I) with β-d-(1 → 3,6)-galactosyl side-chains having terminal β-d-GlcA showed activity in the pectin-enriched fractions. Interestingly, the terminal GlcA was not required for activity of the arabinogalactan-enriched fractions, suggesting at least two different immunomodulating structures.

Original languageEnglish
Pages (from-to)280-293
Number of pages14
JournalPhytochemistry
Volume71
Issue number2-3
DOIs
Publication statusPublished - 2010 Feb

Fingerprint

Galactans
arabinogalactans
galactans
Peyer's Patches
Peyer's patches
immunomodulation
Astragalus
Immunomodulation
Polysaccharides
polysaccharides
pectins
Pectins
galactooligosaccharides
Polymerization
polymerization
1,6-galactan
arabinogalactan
lymphocytes
Antennas
Degradation

Keywords

  • β-d-(1 → 3,6)-Galactan
  • Astragalus mongholics Bunge
  • Enzymatic digestion
  • Exo-β-d-(1 → 3)-galactanase
  • High performance anion-exchange chromatography
  • Immunomodulating activity
  • Intestinal immune system
  • Leguminosae
  • Oligosaccharide side-chain
  • Peyer's patches
  • Polysaccharides

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Horticulture

Cite this

Different contributions of side-chains in β-d-(1 → 3,6)-galactans on intestinal Peyer's patch-immunomodulation by polysaccharides from Astragalus mongholics Bunge. / Kiyohara, Hiroaki; Uchida, Taichi; Takakiwa, Manami; Matsuzaki, Toshiake; Hada, Noriyasu; Takeda, Tadahiro; Shibata, Toshiro; Yamada, Haruki.

In: Phytochemistry, Vol. 71, No. 2-3, 02.2010, p. 280-293.

Research output: Contribution to journalArticle

Kiyohara, Hiroaki ; Uchida, Taichi ; Takakiwa, Manami ; Matsuzaki, Toshiake ; Hada, Noriyasu ; Takeda, Tadahiro ; Shibata, Toshiro ; Yamada, Haruki. / Different contributions of side-chains in β-d-(1 → 3,6)-galactans on intestinal Peyer's patch-immunomodulation by polysaccharides from Astragalus mongholics Bunge. In: Phytochemistry. 2010 ; Vol. 71, No. 2-3. pp. 280-293.
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