Different differentiation kinetics of vascular progenitor cells in primate and mouse embryonic stem cells

Masakatsu Sone, Hiroshi Itoh, Jun Yamashita, Takami Yurugi-Kobayashi, Yutaka Suzuki, Yasushi Kondo, Akane Nonoguchi, Naoki Sawada, Kenichi Yamahara, Kazutoshi Miyashita, Kwijun Park, Masabumi Shibuya, Shinji Nito, Shin Ichi Nishikawa, Kazuwa Nakao

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Background - We demonstrated that vascular endothelial growth factor receptor 2 (VEGF-R2)-positive cells derived from mouse embryonic stem (ES) cells can differentiate into both endothelial cells and mural cells to suffice as vascular progenitor cells (VPCs). Here we examined whether VPCs occur in primate ES cells and investigated the differences in VPC differentiation kinetics between primate and mouse ES cells. Methods and Results - In contrast to mouse ES cells, undifferentiated monkey ES cells expressed VEGF-R2. By culturing these undifferentiated ES cells for 4 days on OP9 feeder layer, VEGF-R2 expression disappeared, and then reappeared after 8 days of differentiation. We then isolated these VEGF-R2-positive and vascular endothelial cadherin (VEcadherin)-negative cells by flow cytometry sorting. Additional 5-day reculture of these VEGF-R2+ VEcadherin- cells on OP9 feeder layer resulted in the appearance of platelet endothelial cell adhesion molecule-1 (PECAM1)-positive, VEcadherin-positive, endothelial nitric oxide synthase (eNOS)-positive endothelial cells. On a collagen IV-coated dish in the presence of serum, these cells differentiated into smooth muscle actin (SMA)-positive and calponin-positive mural cells (pericytes or vascular smooth muscle cells). Addition of 50ng/mL VEGF to the culture on a collagen IV-coated dish resulted in the appearance of PECAM1+ cells surrounded by SMA+ cells. In addition, these differentiated VEGF-R2+ cells can form tube-like structures in a 3-dimensional culture. Conclusion - Our findings indicate that differentiation kinetics of VPCs derived from primate and mouse ES cells were different. Differentiated VEGF-R2+ VEcadherin- cells can act as VPCs in primates. To seek the clinical potential of VPCs for vascular regeneration, investigations of primate ES cells are indispensable.

Original languageEnglish
Pages (from-to)2085-2088
Number of pages4
JournalCirculation
Volume107
Issue number16
DOIs
Publication statusPublished - 2003 Apr 29
Externally publishedYes

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Vascular Endothelial Growth Factor Receptor-2
Primates
Blood Vessels
Stem Cells
Embryonic Stem Cells
Endothelial Cells
CD31 Antigens
Feeder Cells
Smooth Muscle Myocytes
Actins
Collagen
Pericytes
Nitric Oxide Synthase Type III
Mouse Embryonic Stem Cells
Vascular Smooth Muscle
Vascular Endothelial Growth Factor A
Haplorhini
Smooth Muscle
Regeneration
Cell Differentiation

Keywords

  • Angiogenesis
  • Cells
  • Endothelium
  • Muscle, smooth
  • Vessels

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Different differentiation kinetics of vascular progenitor cells in primate and mouse embryonic stem cells. / Sone, Masakatsu; Itoh, Hiroshi; Yamashita, Jun; Yurugi-Kobayashi, Takami; Suzuki, Yutaka; Kondo, Yasushi; Nonoguchi, Akane; Sawada, Naoki; Yamahara, Kenichi; Miyashita, Kazutoshi; Park, Kwijun; Shibuya, Masabumi; Nito, Shinji; Nishikawa, Shin Ichi; Nakao, Kazuwa.

In: Circulation, Vol. 107, No. 16, 29.04.2003, p. 2085-2088.

Research output: Contribution to journalArticle

Sone, M, Itoh, H, Yamashita, J, Yurugi-Kobayashi, T, Suzuki, Y, Kondo, Y, Nonoguchi, A, Sawada, N, Yamahara, K, Miyashita, K, Park, K, Shibuya, M, Nito, S, Nishikawa, SI & Nakao, K 2003, 'Different differentiation kinetics of vascular progenitor cells in primate and mouse embryonic stem cells', Circulation, vol. 107, no. 16, pp. 2085-2088. https://doi.org/10.1161/01.CIR.0000070022.78747.1B
Sone, Masakatsu ; Itoh, Hiroshi ; Yamashita, Jun ; Yurugi-Kobayashi, Takami ; Suzuki, Yutaka ; Kondo, Yasushi ; Nonoguchi, Akane ; Sawada, Naoki ; Yamahara, Kenichi ; Miyashita, Kazutoshi ; Park, Kwijun ; Shibuya, Masabumi ; Nito, Shinji ; Nishikawa, Shin Ichi ; Nakao, Kazuwa. / Different differentiation kinetics of vascular progenitor cells in primate and mouse embryonic stem cells. In: Circulation. 2003 ; Vol. 107, No. 16. pp. 2085-2088.
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AU - Sone, Masakatsu

AU - Itoh, Hiroshi

AU - Yamashita, Jun

AU - Yurugi-Kobayashi, Takami

AU - Suzuki, Yutaka

AU - Kondo, Yasushi

AU - Nonoguchi, Akane

AU - Sawada, Naoki

AU - Yamahara, Kenichi

AU - Miyashita, Kazutoshi

AU - Park, Kwijun

AU - Shibuya, Masabumi

AU - Nito, Shinji

AU - Nishikawa, Shin Ichi

AU - Nakao, Kazuwa

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