TY - JOUR
T1 - Different effects of cytochalasins on the growth and differentiation of Entamoeba invadens
AU - Makioka, Asao
AU - Kumagai, Masahiro
AU - Kobayashi, Seiki
AU - Takeuchi, Tsutomu
N1 - Funding Information:
Acknowledgements We thank Dr. N. Watanabe for valuable discussion, Dr. L.S. Diamond for supplying the Entamoeba invadens and T. Okita for technical assistance. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports and Technology of Japan, and by a Health Science Research Grant for Research on Emerging and Reemerging Infectious Diseases from the Ministry of Health, Labor and Welfare of Japan.
PY - 2004/5
Y1 - 2004/5
N2 - The effect of five different cytochalasins on the growth, encystation and excystation of Entamoeba invadens was examined. At 10 μM, cytochalasins B, D, E and dihydrocytochalasin B markedly inhibited growth. Encystation was inhibited by cytochalasin D at 1 μM but not by other cytochalasins at the same concentration, whereas it was inhibited by 10 μM of cytochalasins B, E and dihydrocytochalasin B as well as cytochalasin D. Excystation, which was assessed by counting the number of metacystic amoebae after inducing excystation, was markedly enhanced by cytochalasin D as previously demonstrated, whereas the enhancing effect of cytochalasins A, B and dihydrocytochalasin B was weak. In contrast, cytochalasin E at 10 μM inhibited excystation and metacystic development. These results indicate that there is a difference in the effect of different cytochalasins on the growth and differentiation of E. invadens, depending on differences in their chemical structure.
AB - The effect of five different cytochalasins on the growth, encystation and excystation of Entamoeba invadens was examined. At 10 μM, cytochalasins B, D, E and dihydrocytochalasin B markedly inhibited growth. Encystation was inhibited by cytochalasin D at 1 μM but not by other cytochalasins at the same concentration, whereas it was inhibited by 10 μM of cytochalasins B, E and dihydrocytochalasin B as well as cytochalasin D. Excystation, which was assessed by counting the number of metacystic amoebae after inducing excystation, was markedly enhanced by cytochalasin D as previously demonstrated, whereas the enhancing effect of cytochalasins A, B and dihydrocytochalasin B was weak. In contrast, cytochalasin E at 10 μM inhibited excystation and metacystic development. These results indicate that there is a difference in the effect of different cytochalasins on the growth and differentiation of E. invadens, depending on differences in their chemical structure.
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U2 - 10.1007/s00436-004-1106-8
DO - 10.1007/s00436-004-1106-8
M3 - Article
C2 - 15103555
AN - SCOPUS:2942704214
SN - 0044-3255
VL - 93
SP - 68
EP - 71
JO - Zeitschrift fur Parasitenkunde
JF - Zeitschrift fur Parasitenkunde
IS - 1
ER -
