Abstract
Aim: The aim of this study is to evaluate the maturation of pulmonary epithelial cells in human fetal lungs at 21 weeks' gestation. Methods: Eight fetuses at 21 weeks' gestation were evaluated. The maturation of pulmonary epithelial cells was assessed by immunohistochemical examination for surfactant proteins and by electron microscopy. Results: Surfactant protein B mature peptide was detected slightly in the epithelial lining of the bronchioles, but was totally absent in the terminal airways. Surfactant protein B proprotein was clearly detected in the epithelial lining of both bronchioles and terminal airways. Transmission electron microscopy of terminal airway cells showed abundant glycogen granules and few intracellular organelles. Conclusions: The production of mature surfactant protein B in terminal airways is scarce at 21 weeks' gestation, which is associated with the immature mechanism of proprotein processing in the cytoplasm.
| Original language | English |
|---|---|
| Pages (from-to) | 500-504 |
| Number of pages | 5 |
| Journal | Pediatrics International |
| Volume | 44 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Human fetus
- Lung maturation
- Pulmonary epithelial cells
- Surfactant protein
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
Cite this
Different expression of surfactant protein B mature peptide and proprotein at 21 weeks' gestation in human fetal pulmonary epithelial cells. / Mori, Kazuhiro; Ikeda, Kazushige; Tanaka, Mamoru.
In: Pediatrics International, Vol. 44, No. 5, 2002, p. 500-504.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Different expression of surfactant protein B mature peptide and proprotein at 21 weeks' gestation in human fetal pulmonary epithelial cells
AU - Mori, Kazuhiro
AU - Ikeda, Kazushige
AU - Tanaka, Mamoru
PY - 2002
Y1 - 2002
N2 - Aim: The aim of this study is to evaluate the maturation of pulmonary epithelial cells in human fetal lungs at 21 weeks' gestation. Methods: Eight fetuses at 21 weeks' gestation were evaluated. The maturation of pulmonary epithelial cells was assessed by immunohistochemical examination for surfactant proteins and by electron microscopy. Results: Surfactant protein B mature peptide was detected slightly in the epithelial lining of the bronchioles, but was totally absent in the terminal airways. Surfactant protein B proprotein was clearly detected in the epithelial lining of both bronchioles and terminal airways. Transmission electron microscopy of terminal airway cells showed abundant glycogen granules and few intracellular organelles. Conclusions: The production of mature surfactant protein B in terminal airways is scarce at 21 weeks' gestation, which is associated with the immature mechanism of proprotein processing in the cytoplasm.
AB - Aim: The aim of this study is to evaluate the maturation of pulmonary epithelial cells in human fetal lungs at 21 weeks' gestation. Methods: Eight fetuses at 21 weeks' gestation were evaluated. The maturation of pulmonary epithelial cells was assessed by immunohistochemical examination for surfactant proteins and by electron microscopy. Results: Surfactant protein B mature peptide was detected slightly in the epithelial lining of the bronchioles, but was totally absent in the terminal airways. Surfactant protein B proprotein was clearly detected in the epithelial lining of both bronchioles and terminal airways. Transmission electron microscopy of terminal airway cells showed abundant glycogen granules and few intracellular organelles. Conclusions: The production of mature surfactant protein B in terminal airways is scarce at 21 weeks' gestation, which is associated with the immature mechanism of proprotein processing in the cytoplasm.
KW - Human fetus
KW - Lung maturation
KW - Pulmonary epithelial cells
KW - Surfactant protein
UR - http://www.scopus.com/inward/record.url?scp=0036381002&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036381002&partnerID=8YFLogxK
U2 - 10.1046/j.1442-200X.2002.01605.x
DO - 10.1046/j.1442-200X.2002.01605.x
M3 - Article
C2 - 12225548
AN - SCOPUS:0036381002
VL - 44
SP - 500
EP - 504
JO - Pediatrics International
JF - Pediatrics International
SN - 1328-8067
IS - 5
ER -