Different patterns in the induction of metallothionein mRNA synthesis among isoforms after acute ethanol administration

Shin Ichi Ono, Yuriko Ishizaki, Ei Ichi Tokuda, Kei Ichi Tabata, Satoru Asami, Takashi Suzuki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The induction of metallothionein (MT) isoform synthesis was investigated in mouse cerebral cortex 18 h after oral ethanol administration. The expression of MT-I isoform mRNA increased in a dose-dependent manner after ethanol loading at doses between 2 g/kg (ethanol/body weight) and 8 g/kg. Lipid peroxide formation, measured as the amount of malondialdehyde- reactive substances, remained at the control level after all of the administered ethanol doses. The expression of MT-III isoform mRNA remained at the control level up until an ethanol loading dose of 4 g/kg and then finally increased to a significant level at a dose of 8 g/kg, which is almost the LD50 for oral ethanol in mice. The different patterns of MT synthesis induction among MT isoforms suggests that the MT-I isoform, which is ubiquitous in mammalian tissues, plays a significant role as an antioxidant. On the other hand, the MT-III isoform, which has a limited tissue distribution, especially in the central nervous system, seems to be implicated in tissue repair and/or protection against critical tissue injury.

Original languageEnglish
Pages (from-to)147-156
Number of pages10
JournalBiological Trace Element Research
Volume115
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

Keywords

  • Antioxidants
  • Metallothionein isoforms
  • Tissue repair

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical
  • Inorganic Chemistry

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