Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines

Kazuhiro Atsukawa, Hidetsugu Saito, Masahiko Takahashi, Yasutada Akiba, Tazuko Ohishi, Hirotoshi Ebinuma, Iwao Kurose, Hiromasa Ishii

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Effects of interleukin-4 (IL-4) and interferon-γ (IFN-γ) on rat Kupffer cell (KC) functions were investigated. Lipopolysaccharide (LPS)-stimulated KCs pretreated with IL-4 produced much more prostaglandin E2 than those pretreated with IFN-γ. In contrast, LPS-stimulated KCs pretreated with IFN-γ produced much more tumor necrosis factor-α, and nitrate and nitrite than those pretreated with IL-4. Different morphologic changes were induced after the culture with each cytokine; transformation of KCs into multinucleated giant cells with IL-4 was especially noted. These results suggest that IFN-γ and IL-4, produced by different subsets of T-helper lymphocytes, differently induce KC into specific morphology, and change KC response to endotoxins, possibly resulting in a modulation of hepatic inflammation.

Original languageEnglish
Pages (from-to)332-338
Number of pages7
JournalInternational Hepatology Communications
Volume6
Issue number6
DOIs
Publication statusPublished - 1997 Mar

Fingerprint

Kupffer Cells
Interleukin-4
Lipopolysaccharides
Interferons
Cytokines
Giant Cells
Nitrites
Helper-Inducer T-Lymphocytes
Dinoprostone
Endotoxins
Nitrates
Tumor Necrosis Factor-alpha
Inflammation
Liver

Keywords

  • interferon-γ
  • interleukin-4
  • nitric oxide
  • prostaglandin E
  • tumor necrosis factor-α

ASJC Scopus subject areas

  • Hepatology

Cite this

Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines. / Atsukawa, Kazuhiro; Saito, Hidetsugu; Takahashi, Masahiko; Akiba, Yasutada; Ohishi, Tazuko; Ebinuma, Hirotoshi; Kurose, Iwao; Ishii, Hiromasa.

In: International Hepatology Communications, Vol. 6, No. 6, 03.1997, p. 332-338.

Research output: Contribution to journalArticle

Atsukawa, Kazuhiro ; Saito, Hidetsugu ; Takahashi, Masahiko ; Akiba, Yasutada ; Ohishi, Tazuko ; Ebinuma, Hirotoshi ; Kurose, Iwao ; Ishii, Hiromasa. / Different response of rat Kupffer cells to lipopolysaccharide after exposure to T-helper cytokines. In: International Hepatology Communications. 1997 ; Vol. 6, No. 6. pp. 332-338.
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