Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis

Junya Yamamoto, Shotaro Kamata, Asumi Miura, Tomoko Nagata, Ryo Kainuma, Isao Ishii

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (. Atrogin-1 and MuRF1), autophagy-lysosome (. LC3b, p62 and Lamp2), amino acid response (. Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (. HO-1 and Gsta1), and amino acid transport (. Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.

Original languageEnglish
Pages (from-to)357-368
Number of pages12
JournalFEBS Open Bio
Volume5
DOIs
Publication statusPublished - 2015 Apr 21

Fingerprint

Fasting
Tissue
Polymerase Chain Reaction
Chemical analysis
Chemical activation
Amino Acids
Thymus
Caloric Restriction
Autophagy
Proteasome Endopeptidase Complex
Ubiquitin
Lysosomes
Liver
Thymus Gland
Small Intestine
Muscle
Testis
Brain
Stomach
Myocardium

Keywords

  • Amino acid response
  • Amino acid transport
  • Autophagy
  • Nrf2
  • Proteasome
  • Ubiquitin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis. / Yamamoto, Junya; Kamata, Shotaro; Miura, Asumi; Nagata, Tomoko; Kainuma, Ryo; Ishii, Isao.

In: FEBS Open Bio, Vol. 5, 21.04.2015, p. 357-368.

Research output: Contribution to journalArticle

Yamamoto, Junya ; Kamata, Shotaro ; Miura, Asumi ; Nagata, Tomoko ; Kainuma, Ryo ; Ishii, Isao. / Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis. In: FEBS Open Bio. 2015 ; Vol. 5. pp. 357-368.
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