Abstract
Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (. Atrogin-1 and MuRF1), autophagy-lysosome (. LC3b, p62 and Lamp2), amino acid response (. Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (. HO-1 and Gsta1), and amino acid transport (. Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.
| Original language | English |
|---|---|
| Pages (from-to) | 357-368 |
| Number of pages | 12 |
| Journal | FEBS Open Bio |
| Volume | 5 |
| DOIs | |
| Publication status | Published - 2015 Apr 21 |
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Keywords
- Amino acid response
- Amino acid transport
- Autophagy
- Nrf2
- Proteasome
- Ubiquitin
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis. / Yamamoto, Junya; Kamata, Shotaro; Miura, Asumi; Nagata, Tomoko; Kainuma, Ryo; Ishii, Isao.
In: FEBS Open Bio, Vol. 5, 21.04.2015, p. 357-368.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis
AU - Yamamoto, Junya
AU - Kamata, Shotaro
AU - Miura, Asumi
AU - Nagata, Tomoko
AU - Kainuma, Ryo
AU - Ishii, Isao
PY - 2015/4/21
Y1 - 2015/4/21
N2 - Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (. Atrogin-1 and MuRF1), autophagy-lysosome (. LC3b, p62 and Lamp2), amino acid response (. Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (. HO-1 and Gsta1), and amino acid transport (. Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.
AB - Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (. Atrogin-1 and MuRF1), autophagy-lysosome (. LC3b, p62 and Lamp2), amino acid response (. Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (. HO-1 and Gsta1), and amino acid transport (. Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.
KW - Amino acid response
KW - Amino acid transport
KW - Autophagy
KW - Nrf2
KW - Proteasome
KW - Ubiquitin
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U2 - 10.1016/j.fob.2015.04.012
DO - 10.1016/j.fob.2015.04.012
M3 - Article
VL - 5
SP - 357
EP - 368
JO - FEBS Open Bio
JF - FEBS Open Bio
SN - 2211-5463
ER -