TY - JOUR
T1 - Differential adaptive responses to 1- or 2-day fasting in various mouse tissues revealed by quantitative PCR analysis
AU - Yamamoto, Junya
AU - Kamata, Shotaro
AU - Miura, Asumi
AU - Nagata, Tomoko
AU - Kainuma, Ryo
AU - Ishii, Isao
N1 - Funding Information:
This study was supported by the Grants-in-Aid for Scientific Research ( 25460072 and 25220103 ) and the Program for Strategic Research Foundation at Private Universities from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan; Keio University Program for the Advancement of Next Generation Research Projects and Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research (to I.I.). I.I. conceived and designed the project; J.Y., S.K., A.M., T.N., and R.K. acquired the data; J.Y., S.K., and I.I. analyzed and interpreted the data; I.I. wrote the paper.
PY - 2015/4/21
Y1 - 2015/4/21
N2 - Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (. Atrogin-1 and MuRF1), autophagy-lysosome (. LC3b, p62 and Lamp2), amino acid response (. Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (. HO-1 and Gsta1), and amino acid transport (. Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.
AB - Dietary or caloric restriction confers various clinical benefits. Short-term fasting of mice is a common experimental procedure that may involve systemic metabolic remodeling, which may significantly affect experimental outputs. This study evaluated adaptive cellular responses after 1- or 2-day fasting in 13 mouse tissues by quantitative PCR using 15 marker primer sets for the activation of ubiquitin-proteasome (. Atrogin-1 and MuRF1), autophagy-lysosome (. LC3b, p62 and Lamp2), amino acid response (. Asns, Trib3, Herpud1, xCT, and Chop), Nrf2-mediated antioxidant (. HO-1 and Gsta1), and amino acid transport (. Slc38a2, Slc7a5, and Slc7a1) systems. Differential activation profiles obtained in seven highly (thymus, liver, spleen, and small intestine) or mildly (stomach, kidney, and colon) atrophied tissues as well as in six non-atrophied tissues (brain, eye, lung, heart, skeletal muscle, and testis) suggested tissue-specific active metabolic remodeling.
KW - Amino acid response
KW - Amino acid transport
KW - Autophagy
KW - Nrf2
KW - Proteasome
KW - Ubiquitin
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U2 - 10.1016/j.fob.2015.04.012
DO - 10.1016/j.fob.2015.04.012
M3 - Article
AN - SCOPUS:84928661784
VL - 5
SP - 357
EP - 368
JO - FEBS Open Bio
JF - FEBS Open Bio
SN - 2211-5463
ER -