Differential cellular localization of antioxidant enzymes in the trigeminal ganglion

H. Sato, M. Shibata, T. Shimizu, S. Shibata, H. Toriumi, T. Ebine, T. Kuroi, T. Iwashita, M. Funakubo, Y. Kayama, C. Akazawa, K. Wajima, T. Nakagawa, H. Okano, N. Suzuki

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Abstract

Because of its high oxygen demands, neural tissue is predisposed to oxidative stress. Here, our aim was to clarify the cellular localization of antioxidant enzymes in the trigeminal ganglion. We found that the transcriptional factor Sox10 is localized exclusively in satellite glial cells (SGCs) in the adult trigeminal ganglion. The use of transgenic mice that express the fluorescent protein Venus under the Sox10 promoter enabled us to distinguish between neurons and SGCs. Although both superoxide dismutases 1 and 2 were present in the neurons, only superoxide dismutase 1 was identified in SGCs. The enzymes relevant to hydrogen peroxide degradation displayed differential cellular localization, such that neurons were endowed with glutathione peroxidase 1 and thioredoxin 2, and catalase and thioredoxin 2 were present in SGCs. Our immunohistochemical finding showed that only SGCs were labeled by the oxidative damage marker 8-hydroxy-2'-deoxyguanosine, which indicates that the antioxidant systems of SGCs were less potent. The transient receptor potential vanilloid subfamily member 1 (TRPV1), the capsaicin receptor, is implicated in inflammatory hyperalgesia, and we demonstrated that topical capsaicin application causes short-lasting mechanical hyperalgesia in the face. Our cell-based assay revealed that TRPV1 agonist stimulation in the presence of TRPV1 overexpression caused reactive oxygen species-mediated caspase-3 activation. Moreover, capsaicin induced the cellular demise of primary TRPV1-positive trigeminal ganglion neurons in a dose-dependent manner, and this effect was inhibited by a free radical scavenger and a pancaspase inhibitor. This study delineates the localization of antioxidative stress-related enzymes in the trigeminal ganglion and reveals the importance of the pivotal role of reactive oxygen species in the TRPV1-mediated caspase-dependent cell death of trigeminal ganglion neurons. Therapeutic measures for antioxidative stress should be taken to prevent damage to trigeminal primary sensory neurons in inflammatory pain disorders.

Original languageEnglish
Pages (from-to)345-358
Number of pages14
JournalNeuroscience
Volume248
DOIs
Publication statusPublished - 2013 Sep 17

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Keywords

  • Catalase
  • Glutathione peroxidase
  • Inflammatory pain
  • Transient receptor potential vanilloid subfamily member 1, caspase
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sato, H., Shibata, M., Shimizu, T., Shibata, S., Toriumi, H., Ebine, T., Kuroi, T., Iwashita, T., Funakubo, M., Kayama, Y., Akazawa, C., Wajima, K., Nakagawa, T., Okano, H., & Suzuki, N. (2013). Differential cellular localization of antioxidant enzymes in the trigeminal ganglion. Neuroscience, 248, 345-358. https://doi.org/10.1016/j.neuroscience.2013.06.010