Differential effect of rebamipide on transmembrane mucin biosynthesis in stratified ocular surface epithelial cells

Yuichi Uchino, Ashley M. Woodward, Pablo Argüeso

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mucins are a group of highly glycosylated glycoproteins responsible for the protection of wet-surfaced epithelia. Recent data indicate that transmembrane mucins differ in their contribution to the protective function of the ocular surface, with MUC16 being the most effective barrier on the apical surface glycocalyx. Here, we investigated the role of the mucoprotective drug rebamipide in the regulation of transmembrane mucin biosynthesis using stratified cultures of human corneal and conjunctival epithelial cells. We find that the addition of rebamipide to corneal, but not conjunctival, epithelial cells increased MUC16 protein biosynthesis. Rebamipide did not affect the levels of MUC1, 4 and 20 compared to control. In these experiments, rebamipide had no effect on the expression levels of Notch intracellular domains, suggesting that the rebamipide-induced increase in MUC16 biosynthesis in differentiated corneal cultures is not regulated by Notch signaling. Overall these findings indicate that rebamipide induces the differential upregulation of MUC16 in stratified cultures of human corneal epithelial cells, which may have implications to the proper restoration of barrier function in ocular surface disease.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalExperimental Eye Research
Volume153
DOIs
Publication statusPublished - 2016 Dec 1
Externally publishedYes

Fingerprint

Mucins
Epithelial Cells
Glycocalyx
Eye Diseases
Protein Biosynthesis
rebamipide
Glycoproteins
Up-Regulation
Epithelium
Pharmaceutical Preparations

Keywords

  • Notch signaling
  • Ocular surface
  • Rebamipide
  • Stratified epithelium
  • Transmembrane mucins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Differential effect of rebamipide on transmembrane mucin biosynthesis in stratified ocular surface epithelial cells. / Uchino, Yuichi; Woodward, Ashley M.; Argüeso, Pablo.

In: Experimental Eye Research, Vol. 153, 01.12.2016, p. 1-7.

Research output: Contribution to journalArticle

@article{24f89d4a2fba4d8cb62e3d85aa619cf6,
title = "Differential effect of rebamipide on transmembrane mucin biosynthesis in stratified ocular surface epithelial cells",
abstract = "Mucins are a group of highly glycosylated glycoproteins responsible for the protection of wet-surfaced epithelia. Recent data indicate that transmembrane mucins differ in their contribution to the protective function of the ocular surface, with MUC16 being the most effective barrier on the apical surface glycocalyx. Here, we investigated the role of the mucoprotective drug rebamipide in the regulation of transmembrane mucin biosynthesis using stratified cultures of human corneal and conjunctival epithelial cells. We find that the addition of rebamipide to corneal, but not conjunctival, epithelial cells increased MUC16 protein biosynthesis. Rebamipide did not affect the levels of MUC1, 4 and 20 compared to control. In these experiments, rebamipide had no effect on the expression levels of Notch intracellular domains, suggesting that the rebamipide-induced increase in MUC16 biosynthesis in differentiated corneal cultures is not regulated by Notch signaling. Overall these findings indicate that rebamipide induces the differential upregulation of MUC16 in stratified cultures of human corneal epithelial cells, which may have implications to the proper restoration of barrier function in ocular surface disease.",
keywords = "Notch signaling, Ocular surface, Rebamipide, Stratified epithelium, Transmembrane mucins",
author = "Yuichi Uchino and Woodward, {Ashley M.} and Pablo Arg{\"u}eso",
year = "2016",
month = "12",
day = "1",
doi = "10.1016/j.exer.2016.10.007",
language = "English",
volume = "153",
pages = "1--7",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Differential effect of rebamipide on transmembrane mucin biosynthesis in stratified ocular surface epithelial cells

AU - Uchino, Yuichi

AU - Woodward, Ashley M.

AU - Argüeso, Pablo

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Mucins are a group of highly glycosylated glycoproteins responsible for the protection of wet-surfaced epithelia. Recent data indicate that transmembrane mucins differ in their contribution to the protective function of the ocular surface, with MUC16 being the most effective barrier on the apical surface glycocalyx. Here, we investigated the role of the mucoprotective drug rebamipide in the regulation of transmembrane mucin biosynthesis using stratified cultures of human corneal and conjunctival epithelial cells. We find that the addition of rebamipide to corneal, but not conjunctival, epithelial cells increased MUC16 protein biosynthesis. Rebamipide did not affect the levels of MUC1, 4 and 20 compared to control. In these experiments, rebamipide had no effect on the expression levels of Notch intracellular domains, suggesting that the rebamipide-induced increase in MUC16 biosynthesis in differentiated corneal cultures is not regulated by Notch signaling. Overall these findings indicate that rebamipide induces the differential upregulation of MUC16 in stratified cultures of human corneal epithelial cells, which may have implications to the proper restoration of barrier function in ocular surface disease.

AB - Mucins are a group of highly glycosylated glycoproteins responsible for the protection of wet-surfaced epithelia. Recent data indicate that transmembrane mucins differ in their contribution to the protective function of the ocular surface, with MUC16 being the most effective barrier on the apical surface glycocalyx. Here, we investigated the role of the mucoprotective drug rebamipide in the regulation of transmembrane mucin biosynthesis using stratified cultures of human corneal and conjunctival epithelial cells. We find that the addition of rebamipide to corneal, but not conjunctival, epithelial cells increased MUC16 protein biosynthesis. Rebamipide did not affect the levels of MUC1, 4 and 20 compared to control. In these experiments, rebamipide had no effect on the expression levels of Notch intracellular domains, suggesting that the rebamipide-induced increase in MUC16 biosynthesis in differentiated corneal cultures is not regulated by Notch signaling. Overall these findings indicate that rebamipide induces the differential upregulation of MUC16 in stratified cultures of human corneal epithelial cells, which may have implications to the proper restoration of barrier function in ocular surface disease.

KW - Notch signaling

KW - Ocular surface

KW - Rebamipide

KW - Stratified epithelium

KW - Transmembrane mucins

UR - http://www.scopus.com/inward/record.url?scp=84992025569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992025569&partnerID=8YFLogxK

U2 - 10.1016/j.exer.2016.10.007

DO - 10.1016/j.exer.2016.10.007

M3 - Article

C2 - 27725198

AN - SCOPUS:84992025569

VL - 153

SP - 1

EP - 7

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

ER -