Differential effects of thyroid hormone on renin secretion, content, and mRNA in juxtaglomerular cells

Atsuhiro Ichihara, Hiroyuki Kobori, Yutaka Miyashita, Matsuhiko Hayashi, Takao Saruta

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29 Citations (Scopus)

Abstract

The effects of thyroid hormone on renin secretion, renin content, and renin mRNA levels in juxtaglomerular (JG) cells harvested from rat kidneys were determined by radioimmunoassays and reverse transcriptase-polymerase chain reaction. Despite a lack of immediate effect, incubation with triiodothyronine dose dependently increased renin secretion during the first 6 h and elevated renin content and renin mRNA levels during the subsequent period. Simultaneous incubation with triiodothyronine and the calcium ionophore A-23187 abolished the increase in renin secretion and attenuated the increase in renin content but did not affect the increase in renin mRNA levels. During simultaneous incubation with triiodothyronine and the adenylate cyclase inhibitor SQ-22536 or membrane-soluble guanosine 3',5'- cyclic monophosphate (cGMP), the increases in renin secretion, content, and mRNA were similar to those observed in the presence of triiodothyronine alone, except for a cGMP-induced attenuation of the increase in renin secretion. These findings suggest that thyroid hormone stimulates renin secretion by JG cells through the calcium-dependent mechanism, whereas the stimulation of renin gene expression by thyroid hormone does not involve intracellular calcium or cyclic nucleotides.

Original languageEnglish
Pages (from-to)E224-E231
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume274
Issue number2 37-2
Publication statusPublished - 1998 Feb 1

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Keywords

  • Adenosine 3',5'-cyclic monophosphate
  • Calcium
  • Guanosine 3',5'-cyclic monophosphate
  • Reverse transcriptase-polymerase chain reaction
  • Triiodothyronine

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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