Differential effects on expression of IL-2 receptors (p55 and p70) by the HTLV-I pX DNA

H. Sabe, A. Tanaka, Haruhiko Siomi, S. Koyasu, S. Yonehara, I. Yahara, Y. Tagaya, K. Sugie, K. Teshigawara, J. Yodoi, M. Hatanaka

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Abstract

Abnormal expression of the low-affinity receptor for interleukin-2 (IL-2R) is a characteristic of the HTLV-I(+) leukemic T cells in adult T-cell leukemia (ATL). Despite the expression of IL-2R bearing Tac antigen (IL-2R/p55), leukemic cells of the majority of ATL patients do not proliferate in response to IL-2. In the human NK cell line, YT, as well as in ATL-derived T cells, the co-expression of IL-2R/p55 and the second IL-2R without the Tac epitope (IL-2R/p70) is required to produce high-affinity IL-2R. To study the effect of HTLV-I on both of the IL-2Rs, we transfected a fragment of HTLV-I containing the p40X gene into YT cells. One of the 2 transfected YT clones (YT/pX-5.1) had an increased level of expression of IL-2R/p55. In contrast, expression of IL-2R/p70 was unaffected, as determined by Scatchard analysis and the cross-linking study using 125I-IL-2. Our results show that the T-cell phenotype is not required for induction of IL-2R/p55 by p40X. We suggest that HTLV-I infection induces a disproportionate induction of IL-2R/p55 without significant enhancement of IL-2R/p70 expression, resulting in the predominant expression of low-affinity IL-2R in ATL. IL-2R/p70 may be a critical parameter determining the IL-2 reactivity of HTLV-I-infected T cells as well as of normal lymphocytes.

Original languageEnglish
Pages (from-to)880-885
Number of pages6
JournalInternational Journal of Cancer
Volume41
Issue number6
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Adult T Cell Leukemia Lymphoma
Human T-lymphotropic virus 1
Interleukin-2 Receptors
Interleukin-2
T-Lymphocytes
DNA
HTLV-I Infections
Natural Killer Cells
Epitopes
Clone Cells
Lymphocytes
Phenotype
Antigens
Cell Line
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sabe, H., Tanaka, A., Siomi, H., Koyasu, S., Yonehara, S., Yahara, I., ... Hatanaka, M. (1988). Differential effects on expression of IL-2 receptors (p55 and p70) by the HTLV-I pX DNA. International Journal of Cancer, 41(6), 880-885.

Differential effects on expression of IL-2 receptors (p55 and p70) by the HTLV-I pX DNA. / Sabe, H.; Tanaka, A.; Siomi, Haruhiko; Koyasu, S.; Yonehara, S.; Yahara, I.; Tagaya, Y.; Sugie, K.; Teshigawara, K.; Yodoi, J.; Hatanaka, M.

In: International Journal of Cancer, Vol. 41, No. 6, 1988, p. 880-885.

Research output: Contribution to journalArticle

Sabe, H, Tanaka, A, Siomi, H, Koyasu, S, Yonehara, S, Yahara, I, Tagaya, Y, Sugie, K, Teshigawara, K, Yodoi, J & Hatanaka, M 1988, 'Differential effects on expression of IL-2 receptors (p55 and p70) by the HTLV-I pX DNA', International Journal of Cancer, vol. 41, no. 6, pp. 880-885.
Sabe, H. ; Tanaka, A. ; Siomi, Haruhiko ; Koyasu, S. ; Yonehara, S. ; Yahara, I. ; Tagaya, Y. ; Sugie, K. ; Teshigawara, K. ; Yodoi, J. ; Hatanaka, M. / Differential effects on expression of IL-2 receptors (p55 and p70) by the HTLV-I pX DNA. In: International Journal of Cancer. 1988 ; Vol. 41, No. 6. pp. 880-885.
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AU - Sabe, H.

AU - Tanaka, A.

AU - Siomi, Haruhiko

AU - Koyasu, S.

AU - Yonehara, S.

AU - Yahara, I.

AU - Tagaya, Y.

AU - Sugie, K.

AU - Teshigawara, K.

AU - Yodoi, J.

AU - Hatanaka, M.

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N2 - Abnormal expression of the low-affinity receptor for interleukin-2 (IL-2R) is a characteristic of the HTLV-I(+) leukemic T cells in adult T-cell leukemia (ATL). Despite the expression of IL-2R bearing Tac antigen (IL-2R/p55), leukemic cells of the majority of ATL patients do not proliferate in response to IL-2. In the human NK cell line, YT, as well as in ATL-derived T cells, the co-expression of IL-2R/p55 and the second IL-2R without the Tac epitope (IL-2R/p70) is required to produce high-affinity IL-2R. To study the effect of HTLV-I on both of the IL-2Rs, we transfected a fragment of HTLV-I containing the p40X gene into YT cells. One of the 2 transfected YT clones (YT/pX-5.1) had an increased level of expression of IL-2R/p55. In contrast, expression of IL-2R/p70 was unaffected, as determined by Scatchard analysis and the cross-linking study using 125I-IL-2. Our results show that the T-cell phenotype is not required for induction of IL-2R/p55 by p40X. We suggest that HTLV-I infection induces a disproportionate induction of IL-2R/p55 without significant enhancement of IL-2R/p70 expression, resulting in the predominant expression of low-affinity IL-2R in ATL. IL-2R/p70 may be a critical parameter determining the IL-2 reactivity of HTLV-I-infected T cells as well as of normal lymphocytes.

AB - Abnormal expression of the low-affinity receptor for interleukin-2 (IL-2R) is a characteristic of the HTLV-I(+) leukemic T cells in adult T-cell leukemia (ATL). Despite the expression of IL-2R bearing Tac antigen (IL-2R/p55), leukemic cells of the majority of ATL patients do not proliferate in response to IL-2. In the human NK cell line, YT, as well as in ATL-derived T cells, the co-expression of IL-2R/p55 and the second IL-2R without the Tac epitope (IL-2R/p70) is required to produce high-affinity IL-2R. To study the effect of HTLV-I on both of the IL-2Rs, we transfected a fragment of HTLV-I containing the p40X gene into YT cells. One of the 2 transfected YT clones (YT/pX-5.1) had an increased level of expression of IL-2R/p55. In contrast, expression of IL-2R/p70 was unaffected, as determined by Scatchard analysis and the cross-linking study using 125I-IL-2. Our results show that the T-cell phenotype is not required for induction of IL-2R/p55 by p40X. We suggest that HTLV-I infection induces a disproportionate induction of IL-2R/p55 without significant enhancement of IL-2R/p70 expression, resulting in the predominant expression of low-affinity IL-2R in ATL. IL-2R/p70 may be a critical parameter determining the IL-2 reactivity of HTLV-I-infected T cells as well as of normal lymphocytes.

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