Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain

Mineko Tomomura, Yuki Hasegawa, Tsutomu Hashikawa, Akito Tomomura, Michisuke Yuzaki, Teiichi Furuichi, Ryoji Yano

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Apoptosis-associated tyrosine kinase (AATYK) is a non-receptor type tyrosine kinase that is predominantly expressed in adult mouse brain. Although it is also expressed in developing brains, its expression pattern and physiological functions are unclear. In the present study, we analyzed expression profiles of AATYK in developing mouse brains and its functional role and subcellular localization in cultured cerebellar granule cells. Expression of AATYK mRNA and protein increased during postnatal brain development. Immunohistochemical analysis indicated that the protein was differentially expressed in postmitotic neurons within various brain areas including the olfactory bulb, cerebral cortex, hippocampus, thalamus, colliculus, cerebellum, and brain stem. Developmental increases in its expression were also observed in cultured cerebellar granule cells. AATYK protein was largely fractionated into the microsomal fraction and was immunocytochemically distributed in an ER-like meshwork of the granule cell soma, suggesting a possible association with the ER membrane. AATYK protein was also present in neurites. In immature granule cells, overexpression of wild-type AATYK promoted neurite outgrowth, whereas that of tyrosine kinase-defective mutant significantly inhibited it. These results suggest that, in addition to its role in cell death in mature neurons, AATYK has a unique role in promoting neurite extension through its tyrosine kinase activity in developing neurons.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalMolecular Brain Research
Volume112
Issue number1-2
DOIs
Publication statusPublished - 2003 Apr 10
Externally publishedYes

Fingerprint

Protein-Tyrosine Kinases
Apoptosis
Brain
Neurites
Neurons
Olfactory Bulb
Carisoprodol
Thalamus
Cerebral Cortex
Cerebellum
Brain Stem
Hippocampus
Cell Death
Messenger RNA
Membranes

Keywords

  • Cerebellar granule cell
  • Immunohistochemistry
  • Neurite outgrowth
  • Tyrosine kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain. / Tomomura, Mineko; Hasegawa, Yuki; Hashikawa, Tsutomu; Tomomura, Akito; Yuzaki, Michisuke; Furuichi, Teiichi; Yano, Ryoji.

In: Molecular Brain Research, Vol. 112, No. 1-2, 10.04.2003, p. 103-112.

Research output: Contribution to journalArticle

Tomomura, Mineko ; Hasegawa, Yuki ; Hashikawa, Tsutomu ; Tomomura, Akito ; Yuzaki, Michisuke ; Furuichi, Teiichi ; Yano, Ryoji. / Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain. In: Molecular Brain Research. 2003 ; Vol. 112, No. 1-2. pp. 103-112.
@article{bf895d0d59ce4c8e9d03f2bf54ab8957,
title = "Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain",
abstract = "Apoptosis-associated tyrosine kinase (AATYK) is a non-receptor type tyrosine kinase that is predominantly expressed in adult mouse brain. Although it is also expressed in developing brains, its expression pattern and physiological functions are unclear. In the present study, we analyzed expression profiles of AATYK in developing mouse brains and its functional role and subcellular localization in cultured cerebellar granule cells. Expression of AATYK mRNA and protein increased during postnatal brain development. Immunohistochemical analysis indicated that the protein was differentially expressed in postmitotic neurons within various brain areas including the olfactory bulb, cerebral cortex, hippocampus, thalamus, colliculus, cerebellum, and brain stem. Developmental increases in its expression were also observed in cultured cerebellar granule cells. AATYK protein was largely fractionated into the microsomal fraction and was immunocytochemically distributed in an ER-like meshwork of the granule cell soma, suggesting a possible association with the ER membrane. AATYK protein was also present in neurites. In immature granule cells, overexpression of wild-type AATYK promoted neurite outgrowth, whereas that of tyrosine kinase-defective mutant significantly inhibited it. These results suggest that, in addition to its role in cell death in mature neurons, AATYK has a unique role in promoting neurite extension through its tyrosine kinase activity in developing neurons.",
keywords = "Cerebellar granule cell, Immunohistochemistry, Neurite outgrowth, Tyrosine kinase",
author = "Mineko Tomomura and Yuki Hasegawa and Tsutomu Hashikawa and Akito Tomomura and Michisuke Yuzaki and Teiichi Furuichi and Ryoji Yano",
year = "2003",
month = "4",
day = "10",
doi = "10.1016/S0169-328X(03)00054-8",
language = "English",
volume = "112",
pages = "103--112",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Differential expression and function of apoptosis-associated tyrosine kinase (AATYK) in the developing mouse brain

AU - Tomomura, Mineko

AU - Hasegawa, Yuki

AU - Hashikawa, Tsutomu

AU - Tomomura, Akito

AU - Yuzaki, Michisuke

AU - Furuichi, Teiichi

AU - Yano, Ryoji

PY - 2003/4/10

Y1 - 2003/4/10

N2 - Apoptosis-associated tyrosine kinase (AATYK) is a non-receptor type tyrosine kinase that is predominantly expressed in adult mouse brain. Although it is also expressed in developing brains, its expression pattern and physiological functions are unclear. In the present study, we analyzed expression profiles of AATYK in developing mouse brains and its functional role and subcellular localization in cultured cerebellar granule cells. Expression of AATYK mRNA and protein increased during postnatal brain development. Immunohistochemical analysis indicated that the protein was differentially expressed in postmitotic neurons within various brain areas including the olfactory bulb, cerebral cortex, hippocampus, thalamus, colliculus, cerebellum, and brain stem. Developmental increases in its expression were also observed in cultured cerebellar granule cells. AATYK protein was largely fractionated into the microsomal fraction and was immunocytochemically distributed in an ER-like meshwork of the granule cell soma, suggesting a possible association with the ER membrane. AATYK protein was also present in neurites. In immature granule cells, overexpression of wild-type AATYK promoted neurite outgrowth, whereas that of tyrosine kinase-defective mutant significantly inhibited it. These results suggest that, in addition to its role in cell death in mature neurons, AATYK has a unique role in promoting neurite extension through its tyrosine kinase activity in developing neurons.

AB - Apoptosis-associated tyrosine kinase (AATYK) is a non-receptor type tyrosine kinase that is predominantly expressed in adult mouse brain. Although it is also expressed in developing brains, its expression pattern and physiological functions are unclear. In the present study, we analyzed expression profiles of AATYK in developing mouse brains and its functional role and subcellular localization in cultured cerebellar granule cells. Expression of AATYK mRNA and protein increased during postnatal brain development. Immunohistochemical analysis indicated that the protein was differentially expressed in postmitotic neurons within various brain areas including the olfactory bulb, cerebral cortex, hippocampus, thalamus, colliculus, cerebellum, and brain stem. Developmental increases in its expression were also observed in cultured cerebellar granule cells. AATYK protein was largely fractionated into the microsomal fraction and was immunocytochemically distributed in an ER-like meshwork of the granule cell soma, suggesting a possible association with the ER membrane. AATYK protein was also present in neurites. In immature granule cells, overexpression of wild-type AATYK promoted neurite outgrowth, whereas that of tyrosine kinase-defective mutant significantly inhibited it. These results suggest that, in addition to its role in cell death in mature neurons, AATYK has a unique role in promoting neurite extension through its tyrosine kinase activity in developing neurons.

KW - Cerebellar granule cell

KW - Immunohistochemistry

KW - Neurite outgrowth

KW - Tyrosine kinase

UR - http://www.scopus.com/inward/record.url?scp=0037430921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037430921&partnerID=8YFLogxK

U2 - 10.1016/S0169-328X(03)00054-8

DO - 10.1016/S0169-328X(03)00054-8

M3 - Article

VL - 112

SP - 103

EP - 112

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -