Differential expression of CCR3 ligand mRNA in guinea pig lungs during allergen-induced inflammation

K. Asano, M. Nakamura, T. Oguma, Koichi Fukunaga, H. Matsubara, T. Shiomi, A. Ishizaka, K. Yamaguchi, M. Kanazawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective and design: The gene expression profile of CCR3 ligands, eotaxin, RANTES, and monocyte chemo-attractant protein-3 (MCP-3), was examined in normal and inflamed guinea pig lungs. Material: Male Hartley guinea pigs (n = 49). Methods: Pulmonary mRNA was obtained from naive animals, animals treated with intravenous lipopolysaccharide administration, and animals repeatedly exposed to aerosolized allergen (ovalbumin). Northern analysis was performed to quantify pulmonary expression of eotaxin, RANTES, and MCP-3 mRNA. Pulmonary eosinophil peroxidase (EPO) activity was measured to quantify eosinophil accumulation. Results: Eotaxin and RANTES mRNAs, but not MCP-3 mRNA, were constitutively expressed in guinea pig lungs. Lipopolysaccharide treatment increased MCP-3 mRNA expression, but not eotaxin or RANTES mRNA. In contrast, allergen exposure in sensitized animals caused an increase in eotaxin mRNA, which demonstrated good temporal and quantitative correlation with pulmonary EPa activity, but not in MCP-3 or RANTES mRNA. Conclusions: Guinea pig CCR3 ligands demonstrated different gene expression profiles in normal and inflamed airways, suggesting that they play different physiological and pathophysiological roles in the airway.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalInflammation Research
Volume50
Issue number12
Publication statusPublished - 2001

Fingerprint

Allergens
Guinea Pigs
Chemokine CCL5
Ligands
Inflammation
Lung
Messenger RNA
Monocytes
Animals
Proteins
Transcriptome
Gene expression
Lipopolysaccharides
Eosinophil Peroxidase
Ovalbumin
Eosinophils
Intravenous Administration

Keywords

  • Asthma
  • Eosinophil
  • Eotaxin
  • Monocyte chemoattractant protein
  • RANTES

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

Asano, K., Nakamura, M., Oguma, T., Fukunaga, K., Matsubara, H., Shiomi, T., ... Kanazawa, M. (2001). Differential expression of CCR3 ligand mRNA in guinea pig lungs during allergen-induced inflammation. Inflammation Research, 50(12), 625-630.

Differential expression of CCR3 ligand mRNA in guinea pig lungs during allergen-induced inflammation. / Asano, K.; Nakamura, M.; Oguma, T.; Fukunaga, Koichi; Matsubara, H.; Shiomi, T.; Ishizaka, A.; Yamaguchi, K.; Kanazawa, M.

In: Inflammation Research, Vol. 50, No. 12, 2001, p. 625-630.

Research output: Contribution to journalArticle

Asano, K, Nakamura, M, Oguma, T, Fukunaga, K, Matsubara, H, Shiomi, T, Ishizaka, A, Yamaguchi, K & Kanazawa, M 2001, 'Differential expression of CCR3 ligand mRNA in guinea pig lungs during allergen-induced inflammation', Inflammation Research, vol. 50, no. 12, pp. 625-630.
Asano, K. ; Nakamura, M. ; Oguma, T. ; Fukunaga, Koichi ; Matsubara, H. ; Shiomi, T. ; Ishizaka, A. ; Yamaguchi, K. ; Kanazawa, M. / Differential expression of CCR3 ligand mRNA in guinea pig lungs during allergen-induced inflammation. In: Inflammation Research. 2001 ; Vol. 50, No. 12. pp. 625-630.
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