Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats

Kei Higuchi, Hisashi Iizasa, Yoshimichi Sai, Satomi Horieya, Kyeong Eun Lee, Masami Wada, Masayuki Deguchi, Tomohiro Nishimura, Tomohiko Wakayama, Atsushi Tamura, Sachiko Tsukita, Noriko Kose, Young Sook Kang, Emi Nakashima

Research output: Contribution to journalArticle

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Abstract

The syncytiotrophoblast, which regulates maternal-fetal transfer of drugs, consists of a single layer in humans, but two layers, i.e., SynI and SynII, in rodents. Polar distribution of transporters in the apical and basal plasma membranes of syncytiotrophoblast is important for placental function in terms of vectorial transport of substrates, but the mechanisms that control protein distribution in the syncytiotrophoblast remain unclear. We have previously established rat syncytiotrophoblast cell lines, TR-TBT 18d-1 and TR-TBT 18d-2, which retain characteristics of SynI and SynII, respectively. In this study, we aimed to characterize the gene expression profiles in the two layers by using these cell lines. DNA microarray analysis indicated that more than 25 mRNAs, including cytoskeleton binding proteins, ezrin and CLP36, are differentially expressed between TR-TBT 18d-1 and TR-TBT 18d-2. Quantitative real time-polymerase chain reaction (PCR) analysis indicated that mRNA expression of ezrin, CLP36, CCN1, and CCN2 is higher in TR-TBT 18d-1 and mRNA expression of elf-1a, hsc70 and flot2 is higher in TR-TBT 18d-2, compared with their counterparts. Immunohistochemical analysis indicated that ezrin is expressed in rat placental villi in vivo, and is located on the apical membranes of TR-TBT 18d-1, while CLP36 is located in the apical and basal sides of TR-TBT 18d-1. The expression of ezrin was highest at gestational days 14 and 18 and was highest among the ezrin/radixin/moesin (ERM) family members. These results may help to clarify the mechanisms controlling polarization of the syncytiotrophoblast and the significance of the double epithelial layers in rat and mouse.

Original languageEnglish
Pages (from-to)1400-1406
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Volume33
Issue number8
DOIs
Publication statusPublished - 2010

Fingerprint

Trophoblasts
Messenger RNA
Chorionic Villi
Cell Line
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Cytoskeleton
Transcriptome
Real-Time Polymerase Chain Reaction
Rodentia
Carrier Proteins
Mothers
Cell Membrane
ezrin
Membranes
Pharmaceutical Preparations
Proteins

Keywords

  • Blood-placenta barrier
  • CLP36
  • Ezrin
  • SynI
  • SynII
  • Trophoblast

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Higuchi, K., Iizasa, H., Sai, Y., Horieya, S., Lee, K. E., Wada, M., ... Nakashima, E. (2010). Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats. Biological and Pharmaceutical Bulletin, 33(8), 1400-1406. https://doi.org/10.1248/bpb.33.1400

Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats. / Higuchi, Kei; Iizasa, Hisashi; Sai, Yoshimichi; Horieya, Satomi; Lee, Kyeong Eun; Wada, Masami; Deguchi, Masayuki; Nishimura, Tomohiro; Wakayama, Tomohiko; Tamura, Atsushi; Tsukita, Sachiko; Kose, Noriko; Kang, Young Sook; Nakashima, Emi.

In: Biological and Pharmaceutical Bulletin, Vol. 33, No. 8, 2010, p. 1400-1406.

Research output: Contribution to journalArticle

Higuchi, K, Iizasa, H, Sai, Y, Horieya, S, Lee, KE, Wada, M, Deguchi, M, Nishimura, T, Wakayama, T, Tamura, A, Tsukita, S, Kose, N, Kang, YS & Nakashima, E 2010, 'Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats', Biological and Pharmaceutical Bulletin, vol. 33, no. 8, pp. 1400-1406. https://doi.org/10.1248/bpb.33.1400
Higuchi, Kei ; Iizasa, Hisashi ; Sai, Yoshimichi ; Horieya, Satomi ; Lee, Kyeong Eun ; Wada, Masami ; Deguchi, Masayuki ; Nishimura, Tomohiro ; Wakayama, Tomohiko ; Tamura, Atsushi ; Tsukita, Sachiko ; Kose, Noriko ; Kang, Young Sook ; Nakashima, Emi. / Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats. In: Biological and Pharmaceutical Bulletin. 2010 ; Vol. 33, No. 8. pp. 1400-1406.
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