Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines

Rina Zakut, Suzanne L. Topalian, Yutaka Kawakami, Marie Mancini, Siona Eliyahu, Steven A. Rosenberg

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The MAGE-1 gene codes for a tumor-specific antigen, MZ2-E, that elicited a cytotoxic T-lymphocyte response in the melanoma patient from whom it was derived. We have developed a simplified method, using polymerase chain reaction amplification of exon 3 followed by restriction enzyme pattern analysis, to distinguish expression of the MAGE-1 gene from MAGE-2 and MAGE-3, other members of this gene family. MAGE-1 mRNA was expressed in 53% of 17 melanoma lines, two of seven Epstein-Barr virus-transformed B-cell lines, and 2 of 5 breast cell lines including a line established from normal breast epithelium. MAGE-1 is not likely to be the common melanoma antigen recognized by the other HLA-A1- or HLA-A2-restricted cytotoxic T-lymphocytes examined in this study, but the fact that it is expressed in about 50% of melanoma cell lines makes it a reasonable target for the immunotherapy of patients bearing HLA-A1.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalCancer Research
Volume53
Issue number1
Publication statusPublished - 1993 Jan 1
Externally publishedYes

Fingerprint

HLA-A1 Antigen
Melanoma
Cytotoxic T-Lymphocytes
Cell Line
Messenger RNA
Breast
Genes
Melanoma-Specific Antigens
HLA-A2 Antigen
Restriction Mapping
Transformed Cell Line
Neoplasm Antigens
Human Herpesvirus 4
Immunotherapy
Exons
B-Lymphocytes
Epithelium
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zakut, R., Topalian, S. L., Kawakami, Y., Mancini, M., Eliyahu, S., & Rosenberg, S. A. (1993). Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines. Cancer Research, 53(1), 5-8.

Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines. / Zakut, Rina; Topalian, Suzanne L.; Kawakami, Yutaka; Mancini, Marie; Eliyahu, Siona; Rosenberg, Steven A.

In: Cancer Research, Vol. 53, No. 1, 01.01.1993, p. 5-8.

Research output: Contribution to journalArticle

Zakut, R, Topalian, SL, Kawakami, Y, Mancini, M, Eliyahu, S & Rosenberg, SA 1993, 'Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines', Cancer Research, vol. 53, no. 1, pp. 5-8.
Zakut R, Topalian SL, Kawakami Y, Mancini M, Eliyahu S, Rosenberg SA. Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines. Cancer Research. 1993 Jan 1;53(1):5-8.
Zakut, Rina ; Topalian, Suzanne L. ; Kawakami, Yutaka ; Mancini, Marie ; Eliyahu, Siona ; Rosenberg, Steven A. / Differential expression of MAGE-1, -2, and -3 messenger RNA in transformed and normal human cell lines. In: Cancer Research. 1993 ; Vol. 53, No. 1. pp. 5-8.
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