Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia

Yoshiki Yagita, Kazuo Kitagawa, Tsutomu Sasaki, Takaki Miyata, Hideyuki Okano, Masatsugu Hori, Masayasu Matsumoto

Research output: Contribution to journalArticle

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Abstract

Both nestin and the neural RNA-binding protein Musashi1 (Msi1) are expressed in neural stem cells in the subventricular zone. Neurogenesis in the hippocampus has received much attention, so we evaluated the expression of Msi1 and nestin in the adult rat hippocampus after transient forebrain ischemia. Both Msi1 and nestin were induced in the reactive astrocytes after ischemia, especially in the CA1 region, until 35 days after ischemia. Induction of both molecules suggested that reactive astrocytes might have immature characteristics. In the subgranular zone (SGZ) of the hippocampal dentate gyrus, Msi1-positive cells formed clusters after ischemia. These cells were labeled by bromodeoxyuridine (BrdU) but did not express glial fibrillary acidic protein. In contrast, very few nestin-positive cells were labeled by BrdU. Our results suggest that neuronal progenitor cells in the SGZ expressed Msi1 but not nestin.

Original languageEnglish
Pages (from-to)750-756
Number of pages7
JournalJournal of Neuroscience Research
Volume69
Issue number6
DOIs
Publication statusPublished - 2002 Sep 15

Fingerprint

Nestin
Hippocampus
Ischemia
Bromodeoxyuridine
Astrocytes
Parahippocampal Gyrus
RNA-Binding Proteins
Neural Stem Cells
Lateral Ventricles
Glial Fibrillary Acidic Protein
Neurogenesis
Dentate Gyrus
Prosencephalon
Stem Cells

Keywords

  • Hippocampus
  • Ischemia
  • Musashi-1 (Msi1)
  • Nestin
  • Neurogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia. / Yagita, Yoshiki; Kitagawa, Kazuo; Sasaki, Tsutomu; Miyata, Takaki; Okano, Hideyuki; Hori, Masatsugu; Matsumoto, Masayasu.

In: Journal of Neuroscience Research, Vol. 69, No. 6, 15.09.2002, p. 750-756.

Research output: Contribution to journalArticle

Yagita, Y, Kitagawa, K, Sasaki, T, Miyata, T, Okano, H, Hori, M & Matsumoto, M 2002, 'Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia', Journal of Neuroscience Research, vol. 69, no. 6, pp. 750-756. https://doi.org/10.1002/jnr.10342
Yagita, Yoshiki ; Kitagawa, Kazuo ; Sasaki, Tsutomu ; Miyata, Takaki ; Okano, Hideyuki ; Hori, Masatsugu ; Matsumoto, Masayasu. / Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia. In: Journal of Neuroscience Research. 2002 ; Vol. 69, No. 6. pp. 750-756.
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