Differential expression of Pax6 and Ngn2 between pair-generated cortical neurons

Ayano Kawaguchi, Masaharu Ogawa, Kanako Saito, Fumio Matsuzaki, Hideyuki Okano, Takaki Miyata

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Progenitor cells that generate neuron pairs ("pair progenitor cells") are implicated in mammalian cortical development, and their division has been thought to be "symmetric." However, asymmetric growth of two sister neurons generated by the division of a pair progenitor cell would lead to more efficient generation of neuronal diversity in the cortex. To explore mechanisms by which pair progenitor cells provide neuronal diversity, we examined molecular differences between a pair of neurons generated in clonal-density culture. Time-course analysis for the acquisition of neuronal markers and the disappearance of Pax6 and Neurogenin2 (Ngn2) demonstrated that 1) these transcription factors are expressed transiently in some but not all young neurons and 2) some neuron pairs showed uneven/asymmetric expression of Pax6 (19.5%) or Ngn2 (23.8%), whereas other pairs were either symmetrically positive or negative. Asymmetric Pax6 distribution in neuron pairs was not associated with asymmetric distribution of Numb, which raises an intriguing possibility, that Pax6 asymmetry in neuron pairs is produced by an alternative mode of the cell autonomous mechanisms. Stage-dependent changes were noted in the pattern of Ngn2 retention in daughter neurons, reflecting qualitative changes in the pair progenitor population. We suggest that pair progenitor cells contribute to the generation of neuronal diversity through cell-intrinsic heterogeneity and asymmetric division.

Original languageEnglish
Pages (from-to)784-795
Number of pages12
JournalJournal of Neuroscience Research
Volume78
Issue number6
DOIs
Publication statusPublished - 2004 Dec 15

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Neurons
Stem Cells
Transcription Factors
Growth
Population

Keywords

  • Asymmetric cell division
  • Clonal culture
  • Cortical development
  • Pair progenitor cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential expression of Pax6 and Ngn2 between pair-generated cortical neurons. / Kawaguchi, Ayano; Ogawa, Masaharu; Saito, Kanako; Matsuzaki, Fumio; Okano, Hideyuki; Miyata, Takaki.

In: Journal of Neuroscience Research, Vol. 78, No. 6, 15.12.2004, p. 784-795.

Research output: Contribution to journalArticle

Kawaguchi, Ayano ; Ogawa, Masaharu ; Saito, Kanako ; Matsuzaki, Fumio ; Okano, Hideyuki ; Miyata, Takaki. / Differential expression of Pax6 and Ngn2 between pair-generated cortical neurons. In: Journal of Neuroscience Research. 2004 ; Vol. 78, No. 6. pp. 784-795.
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