Differential expression of protein kinase C isozymes in rat glial cell cultures

Eliezer Masliah, Kazunari Yoshida, Shun Shimohama, Fred H. Gage, Tsunao Saitoh

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Protein kinase C (PKC) is a family of closely related enzymes implicated in molecular processes involved in growth and differentiation in a variety of cells. We studied the presence and distribution of 4 PKC isozymes in glial cell cultures of the rat hippocampus employing antisera raised against synthetic peptides predicted from the cDNA sequences corresponding to the C-terminal portion of 4 PKC isoforms, α, βI, βII, and γ. PKC(α) and -(βII), but neither PKC(βI) nor -(γ) isoforms were detected in glial cultures of the rat hippocampus. Anti-PKC(α) immunostained all glial cells, whereas anti-PKC(βII) faintly stained about 20% of total glial cells resembling the type-2 astrocyte that were GFAP immunopositive, with few processes. Anti-PKC(βII) did not stain about 80% of the glial fibrillary acidic protein (GFAP)-immunopositive cells with a few thick processes which resembled the type-1 astrocyte. A few cells that stained intensely with anti-PKC(βII) were GFAP immunopositive and possessed fine, but well-developed, multiple processes. Faint PKC(βII) immunoreactivity was also detected among anti-MBP-positive cells (possibly oligodendrocytes), RCA-1-positive cells (possibly microglia), and small, oval, anti-GFAP-positive cells. These results suggest the involvement of distinct PKC isoforms in different glial functions.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalBrain Research
Volume549
Issue number1
DOIs
Publication statusPublished - 1991 May 17
Externally publishedYes

Fingerprint

Neuroglia
Protein Kinase C
Isoenzymes
Cell Culture Techniques
Glial Fibrillary Acidic Protein
Protein Isoforms
Astrocytes
Hippocampus
Oligodendroglia
Microglia
Immune Sera
Coloring Agents
Complementary DNA
Peptides

Keywords

  • Astroglia
  • Glia
  • Microglia
  • Protein kinase C

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Differential expression of protein kinase C isozymes in rat glial cell cultures. / Masliah, Eliezer; Yoshida, Kazunari; Shimohama, Shun; Gage, Fred H.; Saitoh, Tsunao.

In: Brain Research, Vol. 549, No. 1, 17.05.1991, p. 106-111.

Research output: Contribution to journalArticle

Masliah, Eliezer ; Yoshida, Kazunari ; Shimohama, Shun ; Gage, Fred H. ; Saitoh, Tsunao. / Differential expression of protein kinase C isozymes in rat glial cell cultures. In: Brain Research. 1991 ; Vol. 549, No. 1. pp. 106-111.
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