Differential expression of protein kinase C isozymes in rat glial cell cultures

Eliezer Masliah, Kazunari Yoshida, Shun Shimohama, Fred H. Gage, Tsunao Saitoh

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Abstract

Protein kinase C (PKC) is a family of closely related enzymes implicated in molecular processes involved in growth and differentiation in a variety of cells. We studied the presence and distribution of 4 PKC isozymes in glial cell cultures of the rat hippocampus employing antisera raised against synthetic peptides predicted from the cDNA sequences corresponding to the C-terminal portion of 4 PKC isoforms, α, βI, βII, and γ. PKC(α) and -(βII), but neither PKC(βI) nor -(γ) isoforms were detected in glial cultures of the rat hippocampus. Anti-PKC(α) immunostained all glial cells, whereas anti-PKC(βII) faintly stained about 20% of total glial cells resembling the type-2 astrocyte that were GFAP immunopositive, with few processes. Anti-PKC(βII) did not stain about 80% of the glial fibrillary acidic protein (GFAP)-immunopositive cells with a few thick processes which resembled the type-1 astrocyte. A few cells that stained intensely with anti-PKC(βII) were GFAP immunopositive and possessed fine, but well-developed, multiple processes. Faint PKC(βII) immunoreactivity was also detected among anti-MBP-positive cells (possibly oligodendrocytes), RCA-1-positive cells (possibly microglia), and small, oval, anti-GFAP-positive cells. These results suggest the involvement of distinct PKC isoforms in different glial functions.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalBrain Research
Volume549
Issue number1
DOIs
Publication statusPublished - 1991 May 17
Externally publishedYes

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Keywords

  • Astroglia
  • Glia
  • Microglia
  • Protein kinase C

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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