Differential expression of WNTs and FRPs in the synovium of rheumatoid arthritis and osteoarthritis

Kazushi Imai, Masako Morikawa, Jeanine D'Armiento, Hideo Matsumoto, Koichiro Komiya, Yasunori Okada

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Synovial cells of the joint play a key role in the progression of rheumatoid arthritis (RA). However, the mechanism(s) that triggers aggression of RA synovial cells but not other arthropathies such as osteoarthritis (OA) is not clear. Here we examined expression of WNT and the WNT inhibitor, secreted frizzled-related protein (FRP), in RA and OA synovium by reverse transcription-PCR. WNT10B was most frequently detected in RA synovium, and FRP1, FRP2, and FRP4 in OA synovium. Immunohistochemistry localized WNT10B and FRP1 in synovial lining cells, fibroblasts, and endothelial cells in RA and OA synovium, respectively, and WNT10B expression was increased in parallel with the degree of inflammatory cell infiltration and tissue fibrosis. Membrane-type 1 matrix metalloproteinse (MT1MMP) was upregulated by WNT10B and activation of WNT signaling. MT1MMP immunolocalized to cells identical to WNT10B and β-catenin staining. The present study demonstrated that WNTs and FRPs are differentially expressed in RA and OA synovium, and suggests an involvement in the pathology of these diseases.

Original languageEnglish
Pages (from-to)1615-1620
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume345
Issue number4
DOIs
Publication statusPublished - 2006 Jul 14

Fingerprint

Synovial Membrane
Osteoarthritis
Rheumatoid Arthritis
Membranes
Catenins
Endothelial cells
Pathology
Transcription
Fibroblasts
Linings
Infiltration
Chemical activation
Tissue
Joint Diseases
Aggression
Reverse Transcription
Fibrosis
Endothelial Cells
Joints
Immunohistochemistry

Keywords

  • β-Catenin
  • Arthritis
  • FRP
  • Osteoarthritis
  • Rheumatoid arthritis
  • Synovium
  • WNT

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Differential expression of WNTs and FRPs in the synovium of rheumatoid arthritis and osteoarthritis. / Imai, Kazushi; Morikawa, Masako; D'Armiento, Jeanine; Matsumoto, Hideo; Komiya, Koichiro; Okada, Yasunori.

In: Biochemical and Biophysical Research Communications, Vol. 345, No. 4, 14.07.2006, p. 1615-1620.

Research output: Contribution to journalArticle

Imai, Kazushi ; Morikawa, Masako ; D'Armiento, Jeanine ; Matsumoto, Hideo ; Komiya, Koichiro ; Okada, Yasunori. / Differential expression of WNTs and FRPs in the synovium of rheumatoid arthritis and osteoarthritis. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 345, No. 4. pp. 1615-1620.
@article{2528f483ea0f48a788fdb47433abd587,
title = "Differential expression of WNTs and FRPs in the synovium of rheumatoid arthritis and osteoarthritis",
abstract = "Synovial cells of the joint play a key role in the progression of rheumatoid arthritis (RA). However, the mechanism(s) that triggers aggression of RA synovial cells but not other arthropathies such as osteoarthritis (OA) is not clear. Here we examined expression of WNT and the WNT inhibitor, secreted frizzled-related protein (FRP), in RA and OA synovium by reverse transcription-PCR. WNT10B was most frequently detected in RA synovium, and FRP1, FRP2, and FRP4 in OA synovium. Immunohistochemistry localized WNT10B and FRP1 in synovial lining cells, fibroblasts, and endothelial cells in RA and OA synovium, respectively, and WNT10B expression was increased in parallel with the degree of inflammatory cell infiltration and tissue fibrosis. Membrane-type 1 matrix metalloproteinse (MT1MMP) was upregulated by WNT10B and activation of WNT signaling. MT1MMP immunolocalized to cells identical to WNT10B and β-catenin staining. The present study demonstrated that WNTs and FRPs are differentially expressed in RA and OA synovium, and suggests an involvement in the pathology of these diseases.",
keywords = "β-Catenin, Arthritis, FRP, Osteoarthritis, Rheumatoid arthritis, Synovium, WNT",
author = "Kazushi Imai and Masako Morikawa and Jeanine D'Armiento and Hideo Matsumoto and Koichiro Komiya and Yasunori Okada",
year = "2006",
month = "7",
day = "14",
doi = "10.1016/j.bbrc.2006.05.075",
language = "English",
volume = "345",
pages = "1615--1620",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Differential expression of WNTs and FRPs in the synovium of rheumatoid arthritis and osteoarthritis

AU - Imai, Kazushi

AU - Morikawa, Masako

AU - D'Armiento, Jeanine

AU - Matsumoto, Hideo

AU - Komiya, Koichiro

AU - Okada, Yasunori

PY - 2006/7/14

Y1 - 2006/7/14

N2 - Synovial cells of the joint play a key role in the progression of rheumatoid arthritis (RA). However, the mechanism(s) that triggers aggression of RA synovial cells but not other arthropathies such as osteoarthritis (OA) is not clear. Here we examined expression of WNT and the WNT inhibitor, secreted frizzled-related protein (FRP), in RA and OA synovium by reverse transcription-PCR. WNT10B was most frequently detected in RA synovium, and FRP1, FRP2, and FRP4 in OA synovium. Immunohistochemistry localized WNT10B and FRP1 in synovial lining cells, fibroblasts, and endothelial cells in RA and OA synovium, respectively, and WNT10B expression was increased in parallel with the degree of inflammatory cell infiltration and tissue fibrosis. Membrane-type 1 matrix metalloproteinse (MT1MMP) was upregulated by WNT10B and activation of WNT signaling. MT1MMP immunolocalized to cells identical to WNT10B and β-catenin staining. The present study demonstrated that WNTs and FRPs are differentially expressed in RA and OA synovium, and suggests an involvement in the pathology of these diseases.

AB - Synovial cells of the joint play a key role in the progression of rheumatoid arthritis (RA). However, the mechanism(s) that triggers aggression of RA synovial cells but not other arthropathies such as osteoarthritis (OA) is not clear. Here we examined expression of WNT and the WNT inhibitor, secreted frizzled-related protein (FRP), in RA and OA synovium by reverse transcription-PCR. WNT10B was most frequently detected in RA synovium, and FRP1, FRP2, and FRP4 in OA synovium. Immunohistochemistry localized WNT10B and FRP1 in synovial lining cells, fibroblasts, and endothelial cells in RA and OA synovium, respectively, and WNT10B expression was increased in parallel with the degree of inflammatory cell infiltration and tissue fibrosis. Membrane-type 1 matrix metalloproteinse (MT1MMP) was upregulated by WNT10B and activation of WNT signaling. MT1MMP immunolocalized to cells identical to WNT10B and β-catenin staining. The present study demonstrated that WNTs and FRPs are differentially expressed in RA and OA synovium, and suggests an involvement in the pathology of these diseases.

KW - β-Catenin

KW - Arthritis

KW - FRP

KW - Osteoarthritis

KW - Rheumatoid arthritis

KW - Synovium

KW - WNT

UR - http://www.scopus.com/inward/record.url?scp=33744533911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744533911&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.05.075

DO - 10.1016/j.bbrc.2006.05.075

M3 - Article

VL - 345

SP - 1615

EP - 1620

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -