Differential gene expression in response to juvenile hormone analog treatment in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Archotermopsidae)

Richard Cornette, Yoshinobu Hayashi, Shigeyuki Koshikawa, Toru Miura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Termite societies are characterized by a highly organized division of labor among conspicuous castes, groups of individuals with various morphological specializations. Termite caste differentiation is under control of juvenile hormone (JH), but the molecular mechanism underlying the response to JH and early events triggering caste differentiation are still poorly understood. In order to profile candidate gene expression during early soldier caste differentiation of the damp-wood termite, Hodotermopsis sjostedti, we treated pseudergates (workers) with a juvenile hormone analog (JHA) to induce soldier caste differentiation. We then used Suppressive Subtractive Hybridization to create two cDNA libraries enriched for transcripts that were either up- or downregulated at 24. h after treatment. Finally, we used quantitative PCR to confirm temporal expression patterns. Hexamerins represent a large proportion of the genes upregulated following JHA treatment and have an expression pattern that shows roughly an inverse correlation to intrinsic JH titers. This data is consistent with the role of a JH " sink" which was demonstrated for hexamerins in another termite, Reticulitermes flavipes. A putative nuclear protein was also upregulated a few hours after JHA treatment, which suggests a role in the early response to JH and subsequent regulation of transcriptional events associated with soldier caste differentiation. Some digestive enzymes, such as endogenous beta-endoglucanase and chymotrypsin, as well as a protein associated to digestion were identified among genes downregulated after JHA treatment. This suggests that JH may directly influence the pseudergate-specific digestive system.

Original languageEnglish
Pages (from-to)509-518
Number of pages10
JournalJournal of Insect Physiology
Volume59
Issue number4
DOIs
Publication statusPublished - 2013 Apr 1
Externally publishedYes

Fingerprint

Hodotermopsis sjostedti
wood treatment
Isoptera
Juvenile Hormones
juvenile hormone analogs
caste determination
juvenile hormones
Gene Expression
gene expression
Social Class
hexamerins
Military Personnel
Therapeutics
Reticulitermes flavipes
polyethism
suppression subtractive hybridization
nuclear proteins
digestive enzymes
chymotrypsin
endo-1,4-beta-glucanase

Keywords

  • Caste differentiation
  • Gene expression
  • Hexamerins
  • Hodotermopsis sjostedti
  • Juvenile hormone
  • Termite

ASJC Scopus subject areas

  • Physiology
  • Insect Science

Cite this

Differential gene expression in response to juvenile hormone analog treatment in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Archotermopsidae). / Cornette, Richard; Hayashi, Yoshinobu; Koshikawa, Shigeyuki; Miura, Toru.

In: Journal of Insect Physiology, Vol. 59, No. 4, 01.04.2013, p. 509-518.

Research output: Contribution to journalArticle

Cornette, Richard ; Hayashi, Yoshinobu ; Koshikawa, Shigeyuki ; Miura, Toru. / Differential gene expression in response to juvenile hormone analog treatment in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Archotermopsidae). In: Journal of Insect Physiology. 2013 ; Vol. 59, No. 4. pp. 509-518.
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