Differential glycan profiling by lectin microarray targeting tissue specimens

Atsushi Kuno, Atsushi Matsuda, Yuzuru Ikehara, Hisashi Narimatsu, Jun Hirabayashi

Research output: Chapter in Book/Report/Conference proceedingChapter

24 Citations (Scopus)

Abstract

Glycome is defined by the glycosylation machinery with which each cell is equipped, and this differs between species. It is evident that cells show drastic change during cell progression and differentiation associated with tumorigenesis and malformation. Histochemical approaches to analyze molecular and cellular dynamics provide useful clues to answering questions about glycan functions associated with pathology. However, development of glyco-biomarker discovery will require differential glycan analysis in a number of clinical specimens where disease lesions and normal regions from the same tissue sections are compared. In this chapter, we describe a simple but powerful method using an ultrasensitive lectin microarray, which enables rapid and systematic differential glycan analysis targeting restricted regions of formalin-fixed tissue specimens, for example, one-dot sections formatted on tissue arrays. Using this advanced technology followed by an objective statistical analysis, we can select lectin probes to best fit subsequent enrichment procedures to identify target glycoproteins that discriminate diseased and normal regions in the tissue specimens.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages165-179
Number of pages15
EditionC
DOIs
Publication statusPublished - 2010
Externally publishedYes

Publication series

NameMethods in Enzymology
NumberC
Volume478
ISSN (Print)0076-6879

Keywords

  • Biomarker
  • Glycoalteration
  • Lectin microarray
  • Tissue section

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Kuno, A., Matsuda, A., Ikehara, Y., Narimatsu, H., & Hirabayashi, J. (2010). Differential glycan profiling by lectin microarray targeting tissue specimens. In Methods in Enzymology (C ed., pp. 165-179). (Methods in Enzymology; Vol. 478, No. C). Academic Press Inc.. https://doi.org/10.1016/S0076-6879(10)78007-1