Differential glycan profiling by lectin microarray targeting tissue specimens

Atsushi Kuno, Atsushi Matsuda, Yuzuru Ikehara, Hisashi Narimatsu, Jun Hirabayashi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Glycome is defined by the glycosylation machinery with which each cell is equipped, and this differs between species. It is evident that cells show drastic change during cell progression and differentiation associated with tumorigenesis and malformation. Histochemical approaches to analyze molecular and cellular dynamics provide useful clues to answering questions about glycan functions associated with pathology. However, development of glyco-biomarker discovery will require differential glycan analysis in a number of clinical specimens where disease lesions and normal regions from the same tissue sections are compared. In this chapter, we describe a simple but powerful method using an ultrasensitive lectin microarray, which enables rapid and systematic differential glycan analysis targeting restricted regions of formalin-fixed tissue specimens, for example, one-dot sections formatted on tissue arrays. Using this advanced technology followed by an objective statistical analysis, we can select lectin probes to best fit subsequent enrichment procedures to identify target glycoproteins that discriminate diseased and normal regions in the tissue specimens.

Original languageEnglish
Pages (from-to)165-179
Number of pages15
JournalMethods in Enzymology
Volume478
Issue numberC
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Microarrays
Lectins
Polysaccharides
Tissue
Glycosylation
Biomarkers
Pathology
Molecular Dynamics Simulation
Formaldehyde
Machinery
Cell Differentiation
Statistical methods
Glycoproteins
Carcinogenesis
Technology

Keywords

  • Biomarker
  • Glycoalteration
  • Lectin microarray
  • Tissue section

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Differential glycan profiling by lectin microarray targeting tissue specimens. / Kuno, Atsushi; Matsuda, Atsushi; Ikehara, Yuzuru; Narimatsu, Hisashi; Hirabayashi, Jun.

In: Methods in Enzymology, Vol. 478, No. C, 2010, p. 165-179.

Research output: Contribution to journalArticle

Kuno, Atsushi ; Matsuda, Atsushi ; Ikehara, Yuzuru ; Narimatsu, Hisashi ; Hirabayashi, Jun. / Differential glycan profiling by lectin microarray targeting tissue specimens. In: Methods in Enzymology. 2010 ; Vol. 478, No. C. pp. 165-179.
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