Differential induction of cystathionine γ-lyase in the livers and kidneys of mouse dams during gestation and lactation

Noriyuki Akahoshi, Takashi Izumi, Yasuki Ishizaki, Isao Ishii

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cystathionine γ-lyase (CSE) is the last key enzyme in the transsulfuration pathway for the biosynthesis of cysteine from methionine in mammals, and catalyzes the hydrolysis of cystathionine into cysteine. Cysteine can be provided through diet; however, several investigators have suggested that infants may require dietary supplements of cysteine because of very low or undetectable CSE activity in their livers. We have previously shown that CSE levels are much lower in the livers and kidneys of fetal and infant mice than in those of adult mice, suggesting that the maternal supply of cysteine is important for the early development of mice. Here we examined changes of CSE expression in the livers and kidneys of dams during gestation and lactation. Hepatic enlargement was observed as early as gestational day 12.5 (G12.5) and thereafter became more prominent, whereas expression of CSE in the livers was found after postpartum day 1 (P1) and reached a peak at P14. The maintenance of lactation was essential for both hepatic enlargement and CSE expression. In contrast, kidneys gained weight only slightly during lactation while CSE expression in kidneys was markedly induced at G15.5 and then gradually declined through to P28. Serum concentrations of homocysteine (the precursor of cystathionine) were significantly lower in G18.5 dams than in virgins or G15.5 dams, suggesting that the expression of CSE in the kidneys contributes to the effective clearance of homocysteine during the late gestational stage.

Original languageEnglish
Pages (from-to)1799-1802
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number9
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Cystathionine
Lyases
Lactation
Kidney
Pregnancy
Liver
Cysteine
Homocysteine
Dietary Supplements
Methionine
Postpartum Period
Mammals
Hydrolysis
Maintenance
Mothers
Research Personnel

Keywords

  • Cystathionine γ-lyase
  • Cysteine
  • Gestation
  • Homocysteine
  • Lactation
  • Transsulfuration

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Differential induction of cystathionine γ-lyase in the livers and kidneys of mouse dams during gestation and lactation. / Akahoshi, Noriyuki; Izumi, Takashi; Ishizaki, Yasuki; Ishii, Isao.

In: Biological and Pharmaceutical Bulletin, Vol. 29, No. 9, 2006, p. 1799-1802.

Research output: Contribution to journalArticle

Akahoshi, Noriyuki ; Izumi, Takashi ; Ishizaki, Yasuki ; Ishii, Isao. / Differential induction of cystathionine γ-lyase in the livers and kidneys of mouse dams during gestation and lactation. In: Biological and Pharmaceutical Bulletin. 2006 ; Vol. 29, No. 9. pp. 1799-1802.
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