Differential Prevalence of HIV Type 1 Subtype B and CRF01_AE among Different Sexual Transmission Groups in Tokyo, Japan, as Revealed by Subtype-Specific PCR

Shingo Kato, Yuki Saito, Rie Tanaka, Yoshiyuki Hiraishi, Naoto Kitamura, Tomoko Matsumoto, Hideji Hanabusa, Mitsuhiro Kamakura, Yasuo Ikeda, Masayoshi Negishi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We determined the subtype of HIV-1 in 89 infected individuals attending three reference hospitals located in the Tokyo metropolitan area of Japan. Subtyping was performed with subtype-specific polymerase chain reaction (PCR) distinguishing subtype A, B, C, and CRF01_AE and/or phylogenetic analysis of HIV-1 env C2V3C3 sequences. Subtype-specific PCR provided unequivocal results in 97% of samples. Sixty-five subjects were infected with subtype B, 16 with CRF01_AE, 4 with subtype A, 1 with CRF02_AG, and 3 with subtype C. Among 31 Japanese individuals infected through heterosexual contact, 13 were infected with subtype B and 12 with CRF01_AE. All of the 41 Japanese men infected through homosexual contact harbored subtype B. These results indicate that subtype B is exclusively predominant in a homosexually transmitted group, whereas subtype B and CRF01_AE are evenly predominant in a heterosexually transmitted group.

Original languageEnglish
Pages (from-to)1057-1063
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume19
Issue number11
DOIs
Publication statusPublished - 2003 Nov

Fingerprint

Tokyo
HIV-1
Japan
Polymerase Chain Reaction
Heterosexuality
Sexual Minorities

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Differential Prevalence of HIV Type 1 Subtype B and CRF01_AE among Different Sexual Transmission Groups in Tokyo, Japan, as Revealed by Subtype-Specific PCR. / Kato, Shingo; Saito, Yuki; Tanaka, Rie; Hiraishi, Yoshiyuki; Kitamura, Naoto; Matsumoto, Tomoko; Hanabusa, Hideji; Kamakura, Mitsuhiro; Ikeda, Yasuo; Negishi, Masayoshi.

In: AIDS Research and Human Retroviruses, Vol. 19, No. 11, 11.2003, p. 1057-1063.

Research output: Contribution to journalArticle

Kato, S, Saito, Y, Tanaka, R, Hiraishi, Y, Kitamura, N, Matsumoto, T, Hanabusa, H, Kamakura, M, Ikeda, Y & Negishi, M 2003, 'Differential Prevalence of HIV Type 1 Subtype B and CRF01_AE among Different Sexual Transmission Groups in Tokyo, Japan, as Revealed by Subtype-Specific PCR', AIDS Research and Human Retroviruses, vol. 19, no. 11, pp. 1057-1063. https://doi.org/10.1089/088922203322588431
Kato, Shingo ; Saito, Yuki ; Tanaka, Rie ; Hiraishi, Yoshiyuki ; Kitamura, Naoto ; Matsumoto, Tomoko ; Hanabusa, Hideji ; Kamakura, Mitsuhiro ; Ikeda, Yasuo ; Negishi, Masayoshi. / Differential Prevalence of HIV Type 1 Subtype B and CRF01_AE among Different Sexual Transmission Groups in Tokyo, Japan, as Revealed by Subtype-Specific PCR. In: AIDS Research and Human Retroviruses. 2003 ; Vol. 19, No. 11. pp. 1057-1063.
@article{6a2d8fdf37d34f42892e1e91295416dd,
title = "Differential Prevalence of HIV Type 1 Subtype B and CRF01_AE among Different Sexual Transmission Groups in Tokyo, Japan, as Revealed by Subtype-Specific PCR",
abstract = "We determined the subtype of HIV-1 in 89 infected individuals attending three reference hospitals located in the Tokyo metropolitan area of Japan. Subtyping was performed with subtype-specific polymerase chain reaction (PCR) distinguishing subtype A, B, C, and CRF01_AE and/or phylogenetic analysis of HIV-1 env C2V3C3 sequences. Subtype-specific PCR provided unequivocal results in 97{\%} of samples. Sixty-five subjects were infected with subtype B, 16 with CRF01_AE, 4 with subtype A, 1 with CRF02_AG, and 3 with subtype C. Among 31 Japanese individuals infected through heterosexual contact, 13 were infected with subtype B and 12 with CRF01_AE. All of the 41 Japanese men infected through homosexual contact harbored subtype B. These results indicate that subtype B is exclusively predominant in a homosexually transmitted group, whereas subtype B and CRF01_AE are evenly predominant in a heterosexually transmitted group.",
author = "Shingo Kato and Yuki Saito and Rie Tanaka and Yoshiyuki Hiraishi and Naoto Kitamura and Tomoko Matsumoto and Hideji Hanabusa and Mitsuhiro Kamakura and Yasuo Ikeda and Masayoshi Negishi",
year = "2003",
month = "11",
doi = "10.1089/088922203322588431",
language = "English",
volume = "19",
pages = "1057--1063",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "11",

}

TY - JOUR

T1 - Differential Prevalence of HIV Type 1 Subtype B and CRF01_AE among Different Sexual Transmission Groups in Tokyo, Japan, as Revealed by Subtype-Specific PCR

AU - Kato, Shingo

AU - Saito, Yuki

AU - Tanaka, Rie

AU - Hiraishi, Yoshiyuki

AU - Kitamura, Naoto

AU - Matsumoto, Tomoko

AU - Hanabusa, Hideji

AU - Kamakura, Mitsuhiro

AU - Ikeda, Yasuo

AU - Negishi, Masayoshi

PY - 2003/11

Y1 - 2003/11

N2 - We determined the subtype of HIV-1 in 89 infected individuals attending three reference hospitals located in the Tokyo metropolitan area of Japan. Subtyping was performed with subtype-specific polymerase chain reaction (PCR) distinguishing subtype A, B, C, and CRF01_AE and/or phylogenetic analysis of HIV-1 env C2V3C3 sequences. Subtype-specific PCR provided unequivocal results in 97% of samples. Sixty-five subjects were infected with subtype B, 16 with CRF01_AE, 4 with subtype A, 1 with CRF02_AG, and 3 with subtype C. Among 31 Japanese individuals infected through heterosexual contact, 13 were infected with subtype B and 12 with CRF01_AE. All of the 41 Japanese men infected through homosexual contact harbored subtype B. These results indicate that subtype B is exclusively predominant in a homosexually transmitted group, whereas subtype B and CRF01_AE are evenly predominant in a heterosexually transmitted group.

AB - We determined the subtype of HIV-1 in 89 infected individuals attending three reference hospitals located in the Tokyo metropolitan area of Japan. Subtyping was performed with subtype-specific polymerase chain reaction (PCR) distinguishing subtype A, B, C, and CRF01_AE and/or phylogenetic analysis of HIV-1 env C2V3C3 sequences. Subtype-specific PCR provided unequivocal results in 97% of samples. Sixty-five subjects were infected with subtype B, 16 with CRF01_AE, 4 with subtype A, 1 with CRF02_AG, and 3 with subtype C. Among 31 Japanese individuals infected through heterosexual contact, 13 were infected with subtype B and 12 with CRF01_AE. All of the 41 Japanese men infected through homosexual contact harbored subtype B. These results indicate that subtype B is exclusively predominant in a homosexually transmitted group, whereas subtype B and CRF01_AE are evenly predominant in a heterosexually transmitted group.

UR - http://www.scopus.com/inward/record.url?scp=10744230727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744230727&partnerID=8YFLogxK

U2 - 10.1089/088922203322588431

DO - 10.1089/088922203322588431

M3 - Article

C2 - 14686327

AN - SCOPUS:10744230727

VL - 19

SP - 1057

EP - 1063

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 11

ER -