Differential proliferative response in the postischemic hippocampus, temporal cortex, and olfactory bulb of young adult macaque monkeys

Anton B. Tonchev, Tetsumori Yamashima, Liang Zhao, Hideyuki Okano

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We investigated the fate of proliferating cells in the adult monkey brain after global ischemia. We used the thymidine analogue bromodeoxyuridine (BrdU) to label S-phase cells and their progeny in Japanese macaques subjected to global cerebral ischemia for 20 min or to a sham operation. Subsequently, newly generated cells were identified by BrdU immunohistochemistry, and their immunophenotype was determined quantitatively, using specific markers. The ischemic insult significantly increased the number of proliferating cells in the hippocampus and temporal neocortex, where the majority BrdU-labeled cells expressed markers for microglia (Iba1, CD68, and Ham56) or astrocytes (S-100β and glial fibrillary acidic protein [GFAP]). In contrast, the proliferation level in the parahippocampal region remained unchanged. This discrepancy prompted us to investigate the postischemic response in the olfactory bulb, a well-known site of adult cell generation that is anatomically distant from the above-mentioned regions but that is also subjected to the global ischemic insult. The olfactory bulb contained clusters of proliferating cells expressing markers for neural (Musashil and Nestin) and/or neuronal (class III β-tubulin) progenitors; these were immunophenotypically-distinct from other cell types. Their number and distribution were unaltered by ischemia. Our results demonstrate that cell proliferation and differentiation in the adult macaque brain and olfactory bulb are differentially affected by a common insult.

Original languageEnglish
Pages (from-to)209-224
Number of pages16
JournalGLIA
Volume42
Issue number3
DOIs
Publication statusPublished - 2003 May 1

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Olfactory Bulb
Macaca
Temporal Lobe
Haplorhini
Young Adult
Hippocampus
Bromodeoxyuridine
Ischemia
Nestin
Glial Fibrillary Acidic Protein
Neocortex
Brain
Microglia
Tubulin
Brain Ischemia
S Phase
Astrocytes
Thymidine
Cell Differentiation
Cell Count

Keywords

  • Astroglia
  • CA1 sector
  • Global ischemia
  • Microglia
  • Neural progenitor
  • Primate

ASJC Scopus subject areas

  • Immunology

Cite this

Differential proliferative response in the postischemic hippocampus, temporal cortex, and olfactory bulb of young adult macaque monkeys. / Tonchev, Anton B.; Yamashima, Tetsumori; Zhao, Liang; Okano, Hideyuki.

In: GLIA, Vol. 42, No. 3, 01.05.2003, p. 209-224.

Research output: Contribution to journalArticle

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