Differential subcellular localization of neural isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin) in central nervous system neurons

Immunohistochemistry on formalin-fixed paraffin sections employing antigen retrieval by microwave irradiation

Nobuteru Usuda, Hiroyuki Arai, Hidetada Sasaki, Toru Hanai, Tetsuji Nagata, Taro Muramatsu, Randall L. Kincaid, Susumu Higuchi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We examined the immunohistochemical distribution of the two mammalian isoforms of calcineurin catalytic subunits, Aα and Aβ, in central nervous system (CNS) tissues of cows, rats, and humans. Cryostat sections and paraffin sections of paraformaldehyde-fixed tissues were stained with anti-peptide antibodies for each isoform. The same localization pattern was observed in both cryostat and paraffin sections. In the latter, the intensity of the staining was dramatically enhanced by microwave irradiation. Calcineurin isoforms were localized in a variety of nerve cells but not in neuroglial cells. Their differential expression as the Aα isoform in the nucleus and the Aβ isoform in the cytoplasm was present in a variety of CNS nerve cells, most distinctively in Purkinje cells of the cerebellum and pyramidal cells of the cerebrum, irrespective of species. These results suggest that each isoform has distinct intracellular sites of action in CNS neurons and that the phenomenon has been conserved during mammalian evolution.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume44
Issue number1
Publication statusPublished - 1996 Jan
Externally publishedYes

Fingerprint

Calcineurin
Phosphoprotein Phosphatases
Calmodulin
Microwaves
Paraffin
Formaldehyde
Catalytic Domain
Protein Isoforms
Central Nervous System
Immunohistochemistry
Neurons
Antigens
Nerve Tissue
Pyramidal Cells
Purkinje Cells
Cerebrum
Neuroglia
Cerebellum
Anti-Idiotypic Antibodies
Cytoplasm

Keywords

  • Calcineurin, Neurons
  • Catalytic subunit
  • Isoforms
  • Microwave irradiation, Immunohistochemistry

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

@article{9f1c66fae681455cb50c0df40e5a9986,
title = "Differential subcellular localization of neural isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin) in central nervous system neurons: Immunohistochemistry on formalin-fixed paraffin sections employing antigen retrieval by microwave irradiation",
abstract = "We examined the immunohistochemical distribution of the two mammalian isoforms of calcineurin catalytic subunits, Aα and Aβ, in central nervous system (CNS) tissues of cows, rats, and humans. Cryostat sections and paraffin sections of paraformaldehyde-fixed tissues were stained with anti-peptide antibodies for each isoform. The same localization pattern was observed in both cryostat and paraffin sections. In the latter, the intensity of the staining was dramatically enhanced by microwave irradiation. Calcineurin isoforms were localized in a variety of nerve cells but not in neuroglial cells. Their differential expression as the Aα isoform in the nucleus and the Aβ isoform in the cytoplasm was present in a variety of CNS nerve cells, most distinctively in Purkinje cells of the cerebellum and pyramidal cells of the cerebrum, irrespective of species. These results suggest that each isoform has distinct intracellular sites of action in CNS neurons and that the phenomenon has been conserved during mammalian evolution.",
keywords = "Calcineurin, Neurons, Catalytic subunit, Isoforms, Microwave irradiation, Immunohistochemistry",
author = "Nobuteru Usuda and Hiroyuki Arai and Hidetada Sasaki and Toru Hanai and Tetsuji Nagata and Taro Muramatsu and Kincaid, {Randall L.} and Susumu Higuchi",
year = "1996",
month = "1",
language = "English",
volume = "44",
pages = "13--18",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "1",

}

TY - JOUR

T1 - Differential subcellular localization of neural isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin) in central nervous system neurons

T2 - Immunohistochemistry on formalin-fixed paraffin sections employing antigen retrieval by microwave irradiation

AU - Usuda, Nobuteru

AU - Arai, Hiroyuki

AU - Sasaki, Hidetada

AU - Hanai, Toru

AU - Nagata, Tetsuji

AU - Muramatsu, Taro

AU - Kincaid, Randall L.

AU - Higuchi, Susumu

PY - 1996/1

Y1 - 1996/1

N2 - We examined the immunohistochemical distribution of the two mammalian isoforms of calcineurin catalytic subunits, Aα and Aβ, in central nervous system (CNS) tissues of cows, rats, and humans. Cryostat sections and paraffin sections of paraformaldehyde-fixed tissues were stained with anti-peptide antibodies for each isoform. The same localization pattern was observed in both cryostat and paraffin sections. In the latter, the intensity of the staining was dramatically enhanced by microwave irradiation. Calcineurin isoforms were localized in a variety of nerve cells but not in neuroglial cells. Their differential expression as the Aα isoform in the nucleus and the Aβ isoform in the cytoplasm was present in a variety of CNS nerve cells, most distinctively in Purkinje cells of the cerebellum and pyramidal cells of the cerebrum, irrespective of species. These results suggest that each isoform has distinct intracellular sites of action in CNS neurons and that the phenomenon has been conserved during mammalian evolution.

AB - We examined the immunohistochemical distribution of the two mammalian isoforms of calcineurin catalytic subunits, Aα and Aβ, in central nervous system (CNS) tissues of cows, rats, and humans. Cryostat sections and paraffin sections of paraformaldehyde-fixed tissues were stained with anti-peptide antibodies for each isoform. The same localization pattern was observed in both cryostat and paraffin sections. In the latter, the intensity of the staining was dramatically enhanced by microwave irradiation. Calcineurin isoforms were localized in a variety of nerve cells but not in neuroglial cells. Their differential expression as the Aα isoform in the nucleus and the Aβ isoform in the cytoplasm was present in a variety of CNS nerve cells, most distinctively in Purkinje cells of the cerebellum and pyramidal cells of the cerebrum, irrespective of species. These results suggest that each isoform has distinct intracellular sites of action in CNS neurons and that the phenomenon has been conserved during mammalian evolution.

KW - Calcineurin, Neurons

KW - Catalytic subunit

KW - Isoforms

KW - Microwave irradiation, Immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=0030068408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030068408&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 13

EP - 18

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 1

ER -