Differential zonal expression and adrenocorticotropin regulation of secreted protein acidic and rich in cysteine (SPARC), a matricellular protein, in the midgestation human fetal adrenal gland

Implications for adrenal development

Hitoshi Ishimoto, David G. Ginzinger, Tadashi Matsumoto, Yoshihisa Hattori, Masataka Furuya, Kazuhiro Minegishi, Mamoru Tanaka, Yasunori Yoshimura, Robert B. Jaffe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Context: Matricellular proteins are a group of secreted, multifunctional extracellular matrix glycoproteins that includes thrombospondins (TSPs), tenascin-C, and secreted protein acidic and rich in cysteine (SPARC). They may be implicated in the dynamic developmental processes of the human fetal adrenal (HFA) in which the outer, definitive zone (DZ) cells are postulated to proliferate, migrate centripetally, differentiate, and populate the inner, steroidogenic fetal zone (FZ). Objective: The objective of the study was to identify a matricellular molecule that likely plays a major role in HFA development. Design: Studies involved RNA, cryosections, and cell cultures from 14- to 23-wk HFAs and human adult adrenal RNA. Main Outcome Measures: Measures included transcripts encoding matricellular proteins, using real-time quantitative RT-PCR; SPARC localization by immunostaining; and ACTH regulation of SPARC expression and secretion by quantitative RT-PCR and Western blot. Results: SPARC HFA mRNA was 100-, 700-, and 300-fold higher than TSP-1, TSP-2, and tenascin-C mRNA, respectively. HFA SPARC mRNA was 3-fold higher than adult adrenals (P < 0.005), comparable with levels in adult brain (positive control), whereas mRNAs encoding TSP-1 and TSP-2 were lower in fetal than adult adrenals. SPARC immunoreactivity was detected exclusively in the FZ, not DZ. ACTH, a key regulator of HFA growth and function, increased SPARC mRNA (by 1.7-fold at 1 nM, 48h, P < 0.05) in isolated FZ cells but not DZ cells. ACTH up-regulation of SPARC protein was also detected in FZ cell lysates and culture medium. Conclusions: Results suggest a possible role for SPARC in development of functional and/or structural zonation of the HFA.

Original languageEnglish
Pages (from-to)3208-3214
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume91
Issue number8
DOIs
Publication statusPublished - 2006

Fingerprint

Adrenal Glands
Adrenocorticotropic Hormone
Cysteine
Thrombospondins
Proteins
Messenger RNA
Tenascin
Fetal Development
Cell Culture Techniques
RNA
Cell culture
Extracellular Matrix
Culture Media
Real-Time Polymerase Chain Reaction
Brain
Glycoproteins
Up-Regulation
Western Blotting
Outcome Assessment (Health Care)
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Differential zonal expression and adrenocorticotropin regulation of secreted protein acidic and rich in cysteine (SPARC), a matricellular protein, in the midgestation human fetal adrenal gland : Implications for adrenal development. / Ishimoto, Hitoshi; Ginzinger, David G.; Matsumoto, Tadashi; Hattori, Yoshihisa; Furuya, Masataka; Minegishi, Kazuhiro; Tanaka, Mamoru; Yoshimura, Yasunori; Jaffe, Robert B.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 91, No. 8, 2006, p. 3208-3214.

Research output: Contribution to journalArticle

Ishimoto, Hitoshi ; Ginzinger, David G. ; Matsumoto, Tadashi ; Hattori, Yoshihisa ; Furuya, Masataka ; Minegishi, Kazuhiro ; Tanaka, Mamoru ; Yoshimura, Yasunori ; Jaffe, Robert B. / Differential zonal expression and adrenocorticotropin regulation of secreted protein acidic and rich in cysteine (SPARC), a matricellular protein, in the midgestation human fetal adrenal gland : Implications for adrenal development. In: Journal of Clinical Endocrinology and Metabolism. 2006 ; Vol. 91, No. 8. pp. 3208-3214.
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abstract = "Context: Matricellular proteins are a group of secreted, multifunctional extracellular matrix glycoproteins that includes thrombospondins (TSPs), tenascin-C, and secreted protein acidic and rich in cysteine (SPARC). They may be implicated in the dynamic developmental processes of the human fetal adrenal (HFA) in which the outer, definitive zone (DZ) cells are postulated to proliferate, migrate centripetally, differentiate, and populate the inner, steroidogenic fetal zone (FZ). Objective: The objective of the study was to identify a matricellular molecule that likely plays a major role in HFA development. Design: Studies involved RNA, cryosections, and cell cultures from 14- to 23-wk HFAs and human adult adrenal RNA. Main Outcome Measures: Measures included transcripts encoding matricellular proteins, using real-time quantitative RT-PCR; SPARC localization by immunostaining; and ACTH regulation of SPARC expression and secretion by quantitative RT-PCR and Western blot. Results: SPARC HFA mRNA was 100-, 700-, and 300-fold higher than TSP-1, TSP-2, and tenascin-C mRNA, respectively. HFA SPARC mRNA was 3-fold higher than adult adrenals (P < 0.005), comparable with levels in adult brain (positive control), whereas mRNAs encoding TSP-1 and TSP-2 were lower in fetal than adult adrenals. SPARC immunoreactivity was detected exclusively in the FZ, not DZ. ACTH, a key regulator of HFA growth and function, increased SPARC mRNA (by 1.7-fold at 1 nM, 48h, P < 0.05) in isolated FZ cells but not DZ cells. ACTH up-regulation of SPARC protein was also detected in FZ cell lysates and culture medium. Conclusions: Results suggest a possible role for SPARC in development of functional and/or structural zonation of the HFA.",
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T1 - Differential zonal expression and adrenocorticotropin regulation of secreted protein acidic and rich in cysteine (SPARC), a matricellular protein, in the midgestation human fetal adrenal gland

T2 - Implications for adrenal development

AU - Ishimoto, Hitoshi

AU - Ginzinger, David G.

AU - Matsumoto, Tadashi

AU - Hattori, Yoshihisa

AU - Furuya, Masataka

AU - Minegishi, Kazuhiro

AU - Tanaka, Mamoru

AU - Yoshimura, Yasunori

AU - Jaffe, Robert B.

PY - 2006

Y1 - 2006

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AB - Context: Matricellular proteins are a group of secreted, multifunctional extracellular matrix glycoproteins that includes thrombospondins (TSPs), tenascin-C, and secreted protein acidic and rich in cysteine (SPARC). They may be implicated in the dynamic developmental processes of the human fetal adrenal (HFA) in which the outer, definitive zone (DZ) cells are postulated to proliferate, migrate centripetally, differentiate, and populate the inner, steroidogenic fetal zone (FZ). Objective: The objective of the study was to identify a matricellular molecule that likely plays a major role in HFA development. Design: Studies involved RNA, cryosections, and cell cultures from 14- to 23-wk HFAs and human adult adrenal RNA. Main Outcome Measures: Measures included transcripts encoding matricellular proteins, using real-time quantitative RT-PCR; SPARC localization by immunostaining; and ACTH regulation of SPARC expression and secretion by quantitative RT-PCR and Western blot. Results: SPARC HFA mRNA was 100-, 700-, and 300-fold higher than TSP-1, TSP-2, and tenascin-C mRNA, respectively. HFA SPARC mRNA was 3-fold higher than adult adrenals (P < 0.005), comparable with levels in adult brain (positive control), whereas mRNAs encoding TSP-1 and TSP-2 were lower in fetal than adult adrenals. SPARC immunoreactivity was detected exclusively in the FZ, not DZ. ACTH, a key regulator of HFA growth and function, increased SPARC mRNA (by 1.7-fold at 1 nM, 48h, P < 0.05) in isolated FZ cells but not DZ cells. ACTH up-regulation of SPARC protein was also detected in FZ cell lysates and culture medium. Conclusions: Results suggest a possible role for SPARC in development of functional and/or structural zonation of the HFA.

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