Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC‐M and HCC‐T

Hidetsugu Saito, Toshio Morizane, Tetsu Watanabe, Tatehiro Kagawa, Shingo Miyaguchi, Naoki Kumagai, Masaharu Tsuchiya

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51 Citations (Scopus)

Abstract

The in vitro effect of sodium butyrate (SB) on human hepatoma cell lines PLC/PRF/5, HCC‐M and HCC‐T was investigated. SB was added at the non‐toxic but cytostatic concentration of I mM. In all these cell lines, SB reduced cell proliferation and changed the morphology of the cells into a fibroblast‐like shape. In PLC/PRF/5, alpha‐fetoprotein production and c‐myc expression were inhibited. In contrast, gene expression of albumin, one of the normal liver‐cell products, Mid that of integrated hepatitis B virus genome, was increased. In HCC‐M and HCC‐T, c‐myc expression, which was enhanced In the naive state, was reduced. In HCC‐M, fos expression was inhibited but the expression of N‐ and K‐ras genes did not change. SB seemed to induce normal or mature properties of hepatocytes in human hepatoma cell lines.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalInternational Journal of Cancer
Volume48
Issue number2
DOIs
Publication statusPublished - 1991 May 10

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Saito, H., Morizane, T., Watanabe, T., Kagawa, T., Miyaguchi, S., Kumagai, N., & Tsuchiya, M. (1991). Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC‐M and HCC‐T. International Journal of Cancer, 48(2), 291-296. https://doi.org/10.1002/ijc.2910480223