Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T

Hidetsugu Saito, T. Morizane, T. Watanabe, T. Kagawa, S. Miyaguchi, N. Kumagai, M. Tsuchiya

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Abstract

The in vitro effect of sodium butyrate (SB) on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T was investigated. SB was added at the non-toxic but cytostatic concentration of 1 mM. In all these cell lines, SB reduced cell proliferation and changed the morphology of the cells into a fibroblast-like shape. In PLC/PRF/5, alpha-fetoprotein production and c-myc expression were inhibited. In contrast, gene expression of albumin, one of the normal liver-cell products, and that of integrated hepatitis B virus genome, was increased. In HCC-M and HCC-T, c-myc expression, which was enhanced in the naive state, was reduced. In HCC-M, fos expression was inhibited but the expression of N- and K-ras genes did not change. SB seemed to induce normal or mature properties of hepatocytes in human hepatoma cell lines.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalInternational Journal of Cancer
Volume48
Issue number2
Publication statusPublished - 1991

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Butyric Acid
Hepatocellular Carcinoma
Cell Line
ras Genes
alpha-Fetoproteins
Cytostatic Agents
Hepatitis B virus
Hepatocytes
Albumins
Fibroblasts
Cell Proliferation
Genome
Gene Expression
Liver

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Saito, H., Morizane, T., Watanabe, T., Kagawa, T., Miyaguchi, S., Kumagai, N., & Tsuchiya, M. (1991). Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T. International Journal of Cancer, 48(2), 291-296.

Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T. / Saito, Hidetsugu; Morizane, T.; Watanabe, T.; Kagawa, T.; Miyaguchi, S.; Kumagai, N.; Tsuchiya, M.

In: International Journal of Cancer, Vol. 48, No. 2, 1991, p. 291-296.

Research output: Contribution to journalArticle

Saito, H, Morizane, T, Watanabe, T, Kagawa, T, Miyaguchi, S, Kumagai, N & Tsuchiya, M 1991, 'Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T', International Journal of Cancer, vol. 48, no. 2, pp. 291-296.
Saito, Hidetsugu ; Morizane, T. ; Watanabe, T. ; Kagawa, T. ; Miyaguchi, S. ; Kumagai, N. ; Tsuchiya, M. / Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T. In: International Journal of Cancer. 1991 ; Vol. 48, No. 2. pp. 291-296.
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AU - Kumagai, N.

AU - Tsuchiya, M.

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