Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment

Hiroaki Onoe, M. Kato-Negishi, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes differentiation induction of mouse neuronal stem cells (NSCs) in a fiber-shaped three-dimensional (3D) microenvironment created in a core-shell hydrogel micrfiber. NSCs were encapsulated in the hydrogel fiber and induced differentiation by controlling growth factors in a medium. We successfully demonstrated the differentiation of NSCs to neurons and glial cells in the fiber-shaped 3D microenvironment. Interestingly, we found that the spatial configuration of the differentiated neurons is not uniform: neurons were clustered and localized at the surface of the fibers. These results would significantly advance the applicability of fiber-shaped neuronal tissues to medical transplantation, and at the same time, would provide a confined 3D microenvironment as a tool for stem cell biology.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1490-1492
Number of pages3
Volume3
Publication statusPublished - 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Stem cells
Fibers
Neurons
Hydrogels
Cytology
Tissue

Keywords

  • Coaxial laminar flow
  • Differentiation
  • Hydrogel microfiber
  • Microenvironment
  • Neuronal stem cells

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Onoe, H., Kato-Negishi, M., & Takeuchi, S. (2011). Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 3, pp. 1490-1492)

Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment. / Onoe, Hiroaki; Kato-Negishi, M.; Takeuchi, S.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 1490-1492.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Onoe, H, Kato-Negishi, M & Takeuchi, S 2011, Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 1490-1492, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.
Onoe H, Kato-Negishi M, Takeuchi S. Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3. 2011. p. 1490-1492
Onoe, Hiroaki ; Kato-Negishi, M. ; Takeuchi, S. / Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. pp. 1490-1492
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