Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment

H. Onoe, M. Kato-Negishi, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes differentiation induction of mouse neuronal stem cells (NSCs) in a fiber-shaped three-dimensional (3D) microenvironment created in a core-shell hydrogel micrfiber. NSCs were encapsulated in the hydrogel fiber and induced differentiation by controlling growth factors in a medium. We successfully demonstrated the differentiation of NSCs to neurons and glial cells in the fiber-shaped 3D microenvironment. Interestingly, we found that the spatial configuration of the differentiated neurons is not uniform: neurons were clustered and localized at the surface of the fibers. These results would significantly advance the applicability of fiber-shaped neuronal tissues to medical transplantation, and at the same time, would provide a confined 3D microenvironment as a tool for stem cell biology.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1490-1492
Number of pages3
Publication statusPublished - 2011 Dec 1
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Coaxial laminar flow
  • Differentiation
  • Hydrogel microfiber
  • Microenvironment
  • Neuronal stem cells

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Onoe, H., Kato-Negishi, M., & Takeuchi, S. (2011). Differentiation induction of neuronal stem cells in 3D fiber-shaped microenvironment. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1490-1492). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).