Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage

Takashi Kanaya, Kohtaro Miyazawa, Ikuro Takakura, Wataru Itani, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Michael T. Rose, Huw R. McConochie, Hideyuki Okano, Takahiro Yamaguchi, Hisashi Aso

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

M cells are a kind of intestinal epithelial cell in the follicle-associated epithelium of Peyer's patches. These cells can transport antigens and microorganisms into underlying lymphoid tissues. Despite the important role of M cells in mucosal immune responses, the origin and mechanisms of differentiation as well as cell death of M cells remain unclear. To clarify the mechanism of M cell differentiation, we established a novel murine intestinal epithelial cell line (MIE) from the C57BL/6 mouse. MIE cells grow rapidly and have a cobblestone morphology, which is a typical feature of intestinal epithelial cells. Additionally, they express cytokeratin, villin, cell-cell junctional proteins, and alkaline phosphatase activity and can form microvilli. Their expression of Musashi-1 antigen indicates that they may be close to intestinal stem cells or transitamplifying cells. MIE cells are able to differentiate into the M cell lineage following coculture with intestinal lymphocytes, but not with Peyer's patch lymphocytes (PPL). However, PPL costimulated with anti-CD3/CD28 MAbs caused MIE cells to display typical features of Mcells, such as transcytosis activity, the disorganization of microvilli, and the expression of M cell markers. This transcytosis activity of MIE cells was not induced by T cells isolated from PPL costimulated with the same MAbs and was reduced by the depletion of the T cell population from PPL. A mixture of T cells treated with MAbs and B cells both from PPL led MIE cells to differentiate into M cells. We report here that MIE cells have the potential ability to differentiate into M cells and that this differentiation required activated T cells and B cells.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume295
Issue number2
DOIs
Publication statusPublished - 2008 Aug

Fingerprint

Cell Lineage
Epithelial Cells
Cell Line
Peyer's Patches
Lymphocytes
T-Lymphocytes
Transcytosis
Microvilli
Cell Differentiation
B-Lymphocytes
Antigens
Mucosal Immunity
Phosphoprotein Phosphatases
Lymphoid Tissue
Keratins
Coculture Techniques
Inbred C57BL Mouse
Alkaline Phosphatase

Keywords

  • Follicle-associated epithelium
  • M cell
  • Mie cell
  • Peyer's patch
  • Small intestine

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology (medical)
  • Physiology
  • Hepatology

Cite this

Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. / Kanaya, Takashi; Miyazawa, Kohtaro; Takakura, Ikuro; Itani, Wataru; Watanabe, Kouichi; Ohwada, Shyuichi; Kitazawa, Haruki; Rose, Michael T.; McConochie, Huw R.; Okano, Hideyuki; Yamaguchi, Takahiro; Aso, Hisashi.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 295, No. 2, 08.2008.

Research output: Contribution to journalArticle

Kanaya, T, Miyazawa, K, Takakura, I, Itani, W, Watanabe, K, Ohwada, S, Kitazawa, H, Rose, MT, McConochie, HR, Okano, H, Yamaguchi, T & Aso, H 2008, 'Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 295, no. 2. https://doi.org/10.1152/ajpgi.00378.2007
Kanaya, Takashi ; Miyazawa, Kohtaro ; Takakura, Ikuro ; Itani, Wataru ; Watanabe, Kouichi ; Ohwada, Shyuichi ; Kitazawa, Haruki ; Rose, Michael T. ; McConochie, Huw R. ; Okano, Hideyuki ; Yamaguchi, Takahiro ; Aso, Hisashi. / Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2008 ; Vol. 295, No. 2.
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