Differentiation of Entamoeba histolytica from E. dispar facilitated by monoclonal antibodies against a 150-kDa surface antigen

Hiroshi Tachibana, Seikiv Kobayashi, Xun Jia Cheng, Eiji Hiwatashi

Research output: Contribution to journalArticle

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Abstract

Marine monoclonal antibodies (mAbs) were produced against an n-octyl- β-D-glucopyranoside-extracted fraction of trophozoites of Entamoeba histolytica HM-1:IMSS. Four of the mAbs were reactive with a 150-kDa surface antigen characterized by Western-immunoblot analysis under nonreducing conditions. When the reactivity of the four mAbs with nine reference strains of E. histolytica was examined by an indirect fluorescence antibody test, two of the mAbs (EH3015 and EH3023) were found to react with all nine strains and the other two mAbs (EH3056 and EH3126) reacted with seven strains. The four mAbs did not react with any E. dispar reference strain or with other enteric protozoan parasites. The reactivity of EH3015 and EH3023 with numerous isolates of E. histolytica and E. dispar collected in our laboratories was also examined. The 2 mAbs reacted with all of the 37 E. histolytica isolates tested but did not react with any of the 33 isolates of E. dispar. These results indicate that common antigenic epitopes of E. histolytica are on the 150-kDa surface molecule and that mAbs can distinguish between E. histolytica and E. dispar.

Original languageEnglish
Pages (from-to)435-439
Number of pages5
JournalParasitology Research
Volume83
Issue number5
DOIs
Publication statusPublished - 1997

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Entamoeba histolytica
surface antigens
Surface Antigens
monoclonal antibodies
Monoclonal Antibodies
Trophozoites
trophozoites
Entamoeba dispar
epitopes
Protozoa
Epitopes
Parasites
Fluorescence
Western Blotting
fluorescence
parasites
antibodies
Antibodies

ASJC Scopus subject areas

  • Parasitology

Cite this

Differentiation of Entamoeba histolytica from E. dispar facilitated by monoclonal antibodies against a 150-kDa surface antigen. / Tachibana, Hiroshi; Kobayashi, Seikiv; Cheng, Xun Jia; Hiwatashi, Eiji.

In: Parasitology Research, Vol. 83, No. 5, 1997, p. 435-439.

Research output: Contribution to journalArticle

Tachibana, Hiroshi ; Kobayashi, Seikiv ; Cheng, Xun Jia ; Hiwatashi, Eiji. / Differentiation of Entamoeba histolytica from E. dispar facilitated by monoclonal antibodies against a 150-kDa surface antigen. In: Parasitology Research. 1997 ; Vol. 83, No. 5. pp. 435-439.
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