Differentiation of multipotent DFAT cells into smooth muscle-like cells in 3d tubular microenvironment for tissue regeneration applications

Amy Y. Hsiao, Teru Okitsu, Hiroaki Onoe, Mahiro Kiyosawa, Hiroki Teramae, Shintaroh Iwanaga, Shigenori Miura, Tomohiko Kazama, Taro Matsumoto, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes the differentiation of dedifferentiated fat (DFAT) cells cultured within alginate shell microfibers into smooth muscle-like cells. A microfluidic co-axial device was developed and used to fabricate DFAT cell fibers. DFAT cells embedded in extracellular matrix (ECM) composed of fibrin and collagen inside the alginate shell of microfibers were cultured for one week in control and differentiation induction media. Using such technique, we successfully cultured DFAT cells as 3D fibers and demonstrated differentiation of DFAT cells into smooth muscle-like cells. These findings suggest the use of DFAT 3D cell fibers as a promising and efficient approach for tissue regeneration.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1150-1152
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • 3D culture
  • Alginate
  • DFAT
  • Regenerative medicine
  • Smooth muscle cells
  • Tissue engineering

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Hsiao, A. Y., Okitsu, T., Onoe, H., Kiyosawa, M., Teramae, H., Iwanaga, S., Miura, S., Kazama, T., Matsumoto, T., & Takeuchi, S. (2012). Differentiation of multipotent DFAT cells into smooth muscle-like cells in 3d tubular microenvironment for tissue regeneration applications. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1150-1152). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.