Differentiation of neural stem cells regulated by three-dimensional tissue shape

Yuki Matsushiro, Midori Kato-Negishi, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes the effect of three-dimensional (3D) tissue shape on differentiation ratio of neurons. We cultured mouse neural stem cells (mNSCs) in the closed agarose microchamber sealed with an agarose sheet. We succeeded in fabricating mNSC tissues of various 3D shapes in the closed agarose microchamber and induced differentiation of the lane-shaped mNSC tissues to neurons and glial cells. We confirmed that tissues of thin width and thickness have higher differentiation ratio of neurons than that of spherical-shaped tissue, by changing the width and thickness of the lane shaped tissues.

Original languageEnglish
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages285-288
Number of pages4
ISBN (Electronic)9781538627310
DOIs
Publication statusPublished - 2017 Jul 26
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
Duration: 2017 Jun 182017 Jun 22

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan, Province of China
CityKaohsiung
Period17/6/1817/6/22

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Keywords

  • Agarose hydrogel
  • Differentiation induction
  • Micropatterning
  • Stem cell

ASJC Scopus subject areas

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Matsushiro, Y., Kato-Negishi, M., & Onoe, H. (2017). Differentiation of neural stem cells regulated by three-dimensional tissue shape. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 285-288). [7994044] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994044