Differentiation of proliferated NG2-positive glial progenitor cells in a remyelinating lesion

Masahiko Watanabe, Yoshiaki Toyama, Akiko Nishiyama

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

Cells that express the NG2 proteoglycan (NG2+ cells) constitute a large cell population in the adult mammalian central nervous system (CNS). They give rise to mature oligodendrocytes in culture and are thus considered to be oligodendrocyte progenitor cells (OPCs). They proliferate in response to a variety of insults to the CNS, but their ability to differentiate into oligodendrocytes in vivo has not been established. We used bromodeoxyuridine (BrdU) to trace the fate of NG2+ cells that proliferated in response to a chemically induced demyelinating lesion in the adult rat spinal cord. Cells that were proliferating. 24 hr after lesioning were labeled by a single injection of BrdU, and their antigenic phenotype was examined at various times up to 28 days post-lesioning (28 dpl). Initially, at 2 dpl, NG2+/BrdU+ cells were found almost exclusively at the periphery of the lesion. At 7 dpl, the number of NG2+/BrdU+ cells increased in the lesion center and decreased from the surrounding areas. The number of NG2+/BrdU+ cells inside the lesion further decreased with time, concomitant with progression of remyelination and appearance of BrdU+ mature oligodendrocytes. Double labeling with 3H-thymidine and BrdU combined with NG2 immunohistochemistry showed that some NG2+ cells in the lesion had undergone at least two rounds of cell division. These observations strongly suggest that NG2+/BrdU+ cells that appeared in response to the demyelinating insult gave rise to mature remyelinating oligodendrocytes, providing an in vivo evidence for the differentiation of NG2+ cells into oligodendrocytes.

Original languageEnglish
Pages (from-to)826-836
Number of pages11
JournalJournal of Neuroscience Research
Volume69
Issue number6
DOIs
Publication statusPublished - 2002 Sep 15

Fingerprint

Neuroglia
Bromodeoxyuridine
Stem Cells
Oligodendroglia
Central Nervous System
Cell Division
Thymidine
Cell Differentiation
Spinal Cord
Immunohistochemistry
Phenotype
Injections

Keywords

  • BrdU
  • Differentiation
  • NG2
  • Oligodendrocyte
  • Remyelination

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differentiation of proliferated NG2-positive glial progenitor cells in a remyelinating lesion. / Watanabe, Masahiko; Toyama, Yoshiaki; Nishiyama, Akiko.

In: Journal of Neuroscience Research, Vol. 69, No. 6, 15.09.2002, p. 826-836.

Research output: Contribution to journalArticle

Watanabe, Masahiko ; Toyama, Yoshiaki ; Nishiyama, Akiko. / Differentiation of proliferated NG2-positive glial progenitor cells in a remyelinating lesion. In: Journal of Neuroscience Research. 2002 ; Vol. 69, No. 6. pp. 826-836.
@article{8ec7e416e45643c2bddb27f7a28636f8,
title = "Differentiation of proliferated NG2-positive glial progenitor cells in a remyelinating lesion",
abstract = "Cells that express the NG2 proteoglycan (NG2+ cells) constitute a large cell population in the adult mammalian central nervous system (CNS). They give rise to mature oligodendrocytes in culture and are thus considered to be oligodendrocyte progenitor cells (OPCs). They proliferate in response to a variety of insults to the CNS, but their ability to differentiate into oligodendrocytes in vivo has not been established. We used bromodeoxyuridine (BrdU) to trace the fate of NG2+ cells that proliferated in response to a chemically induced demyelinating lesion in the adult rat spinal cord. Cells that were proliferating. 24 hr after lesioning were labeled by a single injection of BrdU, and their antigenic phenotype was examined at various times up to 28 days post-lesioning (28 dpl). Initially, at 2 dpl, NG2+/BrdU+ cells were found almost exclusively at the periphery of the lesion. At 7 dpl, the number of NG2+/BrdU+ cells increased in the lesion center and decreased from the surrounding areas. The number of NG2+/BrdU+ cells inside the lesion further decreased with time, concomitant with progression of remyelination and appearance of BrdU+ mature oligodendrocytes. Double labeling with 3H-thymidine and BrdU combined with NG2 immunohistochemistry showed that some NG2+ cells in the lesion had undergone at least two rounds of cell division. These observations strongly suggest that NG2+/BrdU+ cells that appeared in response to the demyelinating insult gave rise to mature remyelinating oligodendrocytes, providing an in vivo evidence for the differentiation of NG2+ cells into oligodendrocytes.",
keywords = "BrdU, Differentiation, NG2, Oligodendrocyte, Remyelination",
author = "Masahiko Watanabe and Yoshiaki Toyama and Akiko Nishiyama",
year = "2002",
month = "9",
day = "15",
doi = "10.1002/jnr.10338",
language = "English",
volume = "69",
pages = "826--836",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Differentiation of proliferated NG2-positive glial progenitor cells in a remyelinating lesion

AU - Watanabe, Masahiko

AU - Toyama, Yoshiaki

AU - Nishiyama, Akiko

PY - 2002/9/15

Y1 - 2002/9/15

N2 - Cells that express the NG2 proteoglycan (NG2+ cells) constitute a large cell population in the adult mammalian central nervous system (CNS). They give rise to mature oligodendrocytes in culture and are thus considered to be oligodendrocyte progenitor cells (OPCs). They proliferate in response to a variety of insults to the CNS, but their ability to differentiate into oligodendrocytes in vivo has not been established. We used bromodeoxyuridine (BrdU) to trace the fate of NG2+ cells that proliferated in response to a chemically induced demyelinating lesion in the adult rat spinal cord. Cells that were proliferating. 24 hr after lesioning were labeled by a single injection of BrdU, and their antigenic phenotype was examined at various times up to 28 days post-lesioning (28 dpl). Initially, at 2 dpl, NG2+/BrdU+ cells were found almost exclusively at the periphery of the lesion. At 7 dpl, the number of NG2+/BrdU+ cells increased in the lesion center and decreased from the surrounding areas. The number of NG2+/BrdU+ cells inside the lesion further decreased with time, concomitant with progression of remyelination and appearance of BrdU+ mature oligodendrocytes. Double labeling with 3H-thymidine and BrdU combined with NG2 immunohistochemistry showed that some NG2+ cells in the lesion had undergone at least two rounds of cell division. These observations strongly suggest that NG2+/BrdU+ cells that appeared in response to the demyelinating insult gave rise to mature remyelinating oligodendrocytes, providing an in vivo evidence for the differentiation of NG2+ cells into oligodendrocytes.

AB - Cells that express the NG2 proteoglycan (NG2+ cells) constitute a large cell population in the adult mammalian central nervous system (CNS). They give rise to mature oligodendrocytes in culture and are thus considered to be oligodendrocyte progenitor cells (OPCs). They proliferate in response to a variety of insults to the CNS, but their ability to differentiate into oligodendrocytes in vivo has not been established. We used bromodeoxyuridine (BrdU) to trace the fate of NG2+ cells that proliferated in response to a chemically induced demyelinating lesion in the adult rat spinal cord. Cells that were proliferating. 24 hr after lesioning were labeled by a single injection of BrdU, and their antigenic phenotype was examined at various times up to 28 days post-lesioning (28 dpl). Initially, at 2 dpl, NG2+/BrdU+ cells were found almost exclusively at the periphery of the lesion. At 7 dpl, the number of NG2+/BrdU+ cells increased in the lesion center and decreased from the surrounding areas. The number of NG2+/BrdU+ cells inside the lesion further decreased with time, concomitant with progression of remyelination and appearance of BrdU+ mature oligodendrocytes. Double labeling with 3H-thymidine and BrdU combined with NG2 immunohistochemistry showed that some NG2+ cells in the lesion had undergone at least two rounds of cell division. These observations strongly suggest that NG2+/BrdU+ cells that appeared in response to the demyelinating insult gave rise to mature remyelinating oligodendrocytes, providing an in vivo evidence for the differentiation of NG2+ cells into oligodendrocytes.

KW - BrdU

KW - Differentiation

KW - NG2

KW - Oligodendrocyte

KW - Remyelination

UR - http://www.scopus.com/inward/record.url?scp=0037106212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037106212&partnerID=8YFLogxK

U2 - 10.1002/jnr.10338

DO - 10.1002/jnr.10338

M3 - Article

C2 - 12205676

AN - SCOPUS:0037106212

VL - 69

SP - 826

EP - 836

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 6

ER -