Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics

Yohei Bamba, Tomoko Shofuda, Daisuke Kanematsu, Masahiro Nonaka, Mami Yamasaki, Hideyuki Okano, Yonehiro Kanemura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.

Original languageEnglish
Pages (from-to)683-688
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume447
Issue number4
DOIs
Publication statusPublished - 2014 May 16

Fingerprint

Induced Pluripotent Stem Cells
Neurites
Stem cells
Neuroglia
Stem Cells
Polarization
Cell Line
Scaffolds
Regenerative Medicine
Glioma

Keywords

  • Glioma
  • Induced pluripotent stem cells
  • Neuronal migration
  • PiggyBac transposon
  • Radial glia
  • Regenerative medicine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. / Bamba, Yohei; Shofuda, Tomoko; Kanematsu, Daisuke; Nonaka, Masahiro; Yamasaki, Mami; Okano, Hideyuki; Kanemura, Yonehiro.

In: Biochemical and Biophysical Research Communications, Vol. 447, No. 4, 16.05.2014, p. 683-688.

Research output: Contribution to journalArticle

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