Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation

Amane Kobayashi, Yuki Takayama, Koji Okajima, Mao Oide, Takahiro Yamamoto, Yuki Sekiguchi, Tomotaka Oroguchi, Masayoshi Nakasako, Yoshiki Kohmura, Masaki Yamamoto, Takahiko Hoshi, Yasufumi Torizuka

Research output: Contribution to journalArticle

Abstract

X-ray diffraction imaging is a technique for visualizing the structure of biological cells. In X-ray diffraction imaging experiments using synchrotron radiation, cryogenic conditions are necessary in order to reduce radiation damage in the biological cells. Frozen-hydrated biological specimens kept at cryogenic temperatures are also free from drying and bubbling, which occurs in wet specimens under vacuum conditions. In a previous study, the diffraction apparatus KOTOBUKI-1 [Nakasako et al. (2013), Rev. Sci. Instrum. 84, 093705] was constructed for X-ray diffraction imaging at cryogenic temperatures by utilizing a cryogenic pot, which is a cooling device developed in low-temperature physics. In this study a new cryogenic pot, suitable for tomography experiments, has been developed. The pot can rotate a biological cell over an angular range of ±170° against the direction of the incident X-ray beam. Herein, the details and the performance of the pot and miscellaneous devices are reported, along with established experimental procedures including specimen preparation. The apparatus has been used in tomography experiments for visualizing the three-dimensional structure of a Cyanidioschyzon merolae cell with an approximate size of 5 µm at a resolution of 136 nm. Based on the experimental results, the necessary improvements for future experiments and the resolution limit achievable under experimental conditions within a maximum tolerable dose are discussed.

Original languageEnglish
JournalJournal of Synchrotron Radiation
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

cryogenic temperature
Synchrotrons
Cryogenics
Tomography
synchrotrons
tomography
Diffraction
cryogenics
Imaging techniques
Radiation
X ray diffraction
X rays
radiation
diffraction
x rays
low temperature physics
Temperature
Experiments
Specimen preparation
radiation damage

Keywords

  • biological cells
  • cryogenic experiments
  • tomography
  • X-ray diffraction imaging

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

Cite this

Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation. / Kobayashi, Amane; Takayama, Yuki; Okajima, Koji; Oide, Mao; Yamamoto, Takahiro; Sekiguchi, Yuki; Oroguchi, Tomotaka; Nakasako, Masayoshi; Kohmura, Yoshiki; Yamamoto, Masaki; Hoshi, Takahiko; Torizuka, Yasufumi.

In: Journal of Synchrotron Radiation, 01.01.2018.

Research output: Contribution to journalArticle

Kobayashi, Amane ; Takayama, Yuki ; Okajima, Koji ; Oide, Mao ; Yamamoto, Takahiro ; Sekiguchi, Yuki ; Oroguchi, Tomotaka ; Nakasako, Masayoshi ; Kohmura, Yoshiki ; Yamamoto, Masaki ; Hoshi, Takahiko ; Torizuka, Yasufumi. / Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation. In: Journal of Synchrotron Radiation. 2018.
@article{75995719bb894a19a4b1a887e26720d3,
title = "Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation",
abstract = "X-ray diffraction imaging is a technique for visualizing the structure of biological cells. In X-ray diffraction imaging experiments using synchrotron radiation, cryogenic conditions are necessary in order to reduce radiation damage in the biological cells. Frozen-hydrated biological specimens kept at cryogenic temperatures are also free from drying and bubbling, which occurs in wet specimens under vacuum conditions. In a previous study, the diffraction apparatus KOTOBUKI-1 [Nakasako et al. (2013), Rev. Sci. Instrum. 84, 093705] was constructed for X-ray diffraction imaging at cryogenic temperatures by utilizing a cryogenic pot, which is a cooling device developed in low-temperature physics. In this study a new cryogenic pot, suitable for tomography experiments, has been developed. The pot can rotate a biological cell over an angular range of ±170° against the direction of the incident X-ray beam. Herein, the details and the performance of the pot and miscellaneous devices are reported, along with established experimental procedures including specimen preparation. The apparatus has been used in tomography experiments for visualizing the three-dimensional structure of a Cyanidioschyzon merolae cell with an approximate size of 5 µm at a resolution of 136 nm. Based on the experimental results, the necessary improvements for future experiments and the resolution limit achievable under experimental conditions within a maximum tolerable dose are discussed.",
keywords = "biological cells, cryogenic experiments, tomography, X-ray diffraction imaging",
author = "Amane Kobayashi and Yuki Takayama and Koji Okajima and Mao Oide and Takahiro Yamamoto and Yuki Sekiguchi and Tomotaka Oroguchi and Masayoshi Nakasako and Yoshiki Kohmura and Masaki Yamamoto and Takahiko Hoshi and Yasufumi Torizuka",
year = "2018",
month = "1",
day = "1",
doi = "10.1107/S1600577518012687",
language = "English",
journal = "Journal of Synchrotron Radiation",
issn = "0909-0495",
publisher = "International Union of Crystallography",

}

TY - JOUR

T1 - Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation

AU - Kobayashi, Amane

AU - Takayama, Yuki

AU - Okajima, Koji

AU - Oide, Mao

AU - Yamamoto, Takahiro

AU - Sekiguchi, Yuki

AU - Oroguchi, Tomotaka

AU - Nakasako, Masayoshi

AU - Kohmura, Yoshiki

AU - Yamamoto, Masaki

AU - Hoshi, Takahiko

AU - Torizuka, Yasufumi

PY - 2018/1/1

Y1 - 2018/1/1

N2 - X-ray diffraction imaging is a technique for visualizing the structure of biological cells. In X-ray diffraction imaging experiments using synchrotron radiation, cryogenic conditions are necessary in order to reduce radiation damage in the biological cells. Frozen-hydrated biological specimens kept at cryogenic temperatures are also free from drying and bubbling, which occurs in wet specimens under vacuum conditions. In a previous study, the diffraction apparatus KOTOBUKI-1 [Nakasako et al. (2013), Rev. Sci. Instrum. 84, 093705] was constructed for X-ray diffraction imaging at cryogenic temperatures by utilizing a cryogenic pot, which is a cooling device developed in low-temperature physics. In this study a new cryogenic pot, suitable for tomography experiments, has been developed. The pot can rotate a biological cell over an angular range of ±170° against the direction of the incident X-ray beam. Herein, the details and the performance of the pot and miscellaneous devices are reported, along with established experimental procedures including specimen preparation. The apparatus has been used in tomography experiments for visualizing the three-dimensional structure of a Cyanidioschyzon merolae cell with an approximate size of 5 µm at a resolution of 136 nm. Based on the experimental results, the necessary improvements for future experiments and the resolution limit achievable under experimental conditions within a maximum tolerable dose are discussed.

AB - X-ray diffraction imaging is a technique for visualizing the structure of biological cells. In X-ray diffraction imaging experiments using synchrotron radiation, cryogenic conditions are necessary in order to reduce radiation damage in the biological cells. Frozen-hydrated biological specimens kept at cryogenic temperatures are also free from drying and bubbling, which occurs in wet specimens under vacuum conditions. In a previous study, the diffraction apparatus KOTOBUKI-1 [Nakasako et al. (2013), Rev. Sci. Instrum. 84, 093705] was constructed for X-ray diffraction imaging at cryogenic temperatures by utilizing a cryogenic pot, which is a cooling device developed in low-temperature physics. In this study a new cryogenic pot, suitable for tomography experiments, has been developed. The pot can rotate a biological cell over an angular range of ±170° against the direction of the incident X-ray beam. Herein, the details and the performance of the pot and miscellaneous devices are reported, along with established experimental procedures including specimen preparation. The apparatus has been used in tomography experiments for visualizing the three-dimensional structure of a Cyanidioschyzon merolae cell with an approximate size of 5 µm at a resolution of 136 nm. Based on the experimental results, the necessary improvements for future experiments and the resolution limit achievable under experimental conditions within a maximum tolerable dose are discussed.

KW - biological cells

KW - cryogenic experiments

KW - tomography

KW - X-ray diffraction imaging

UR - http://www.scopus.com/inward/record.url?scp=85055322378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055322378&partnerID=8YFLogxK

U2 - 10.1107/S1600577518012687

DO - 10.1107/S1600577518012687

M3 - Article

C2 - 30407193

AN - SCOPUS:85055322378

JO - Journal of Synchrotron Radiation

JF - Journal of Synchrotron Radiation

SN - 0909-0495

ER -